PMID- 17688944
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20220310
IS  - 0143-4179 (Print)
IS  - 0143-4179 (Linking)
VI  - 41
IP  - 5
DP  - 2007 Oct
TI  - Alterations of BDNF and NT-3 genes expression in the nucleus 
      paragigantocellularis during morphine dependency and withdrawal.
PG  - 321-8
AB  - Locus coeruleus (LC) plays a key role in opioid dependence and withdrawal. 
      Chronic morphine administration induces neurochemical adaptations in the 
      noradrenergic system. The nature of signal responsible for opiate-induced 
      adaptations of noradrenergic neurons in LC is not well defined. 
      Neurotrophins-signaling pathways such as brain derived neurotrophic factor (BDNF) 
      and Neurotrophin-3 (NT-3) play a key role for regulating the noradrenergic 
      response of LC neurons to opiates. The nucleus paragigantocellularis (PGi) is one 
      of the two major afferents to LC. The present study was designed to evaluate the 
      expression of BDNF and NT-3 in the context of opiate dependence and withdrawal in 
      PGi. Such data are important because they could reveal the role of PGi as an 
      additional source of BDNF and NT-3 in the neurochemical plasticity of LC neurons. 
      Opiate dependence was induced by a progressive intraperitoneal treatment of 
      morphine. In morphine dependent group PGi nucleus was extracted for gene 
      expression assay 6h after the last injection of morphine. In spontaneous 
      withdrawal, rats received the same chronic treatment as morphine group. PGi was 
      extracted for gene expression assay 24, 48 and 72 h after the last injection of 
      morphine. PGi nucleus was assayed for the expression of BDNF and NT-3 using 
      semi-quantitative RT-PCR normalized to beta-actin gene expression. Results showed 
      that chronic administration of morphine significantly increased BDNF and NT-3 
      gene expression in PGi. In spontaneous withdrawal, BDNF/NT-3 genes expression 
      were high in comparison to control group. It seems that BDNF/NT-3 -signaling 
      pathway originating from PGi is essential for opiate-induced adaptations of the 
      LC neurons.
FAU - Hatami, Homeira
AU  - Hatami H
AD  - Department of Biology, Teacher Training University, Tehran, Iran.
FAU - Oryan, Shahrbanoo
AU  - Oryan S
FAU - Semnanian, Saeed
AU  - Semnanian S
FAU - Kazemi, Bahram
AU  - Kazemi B
FAU - Bandepour, Mojgan
AU  - Bandepour M
FAU - Ahmadiani, Abolhassan
AU  - Ahmadiani A
LA  - eng
PT  - Journal Article
DEP - 20070803
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 57657-35-9 (neurotropin)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - DNA Primers
MH  - Gene Expression Regulation/*drug effects
MH  - Locus Coeruleus/drug effects/physiopathology
MH  - Male
MH  - Morphine Dependence/*genetics
MH  - Polysaccharides/*genetics
MH  - RNA/genetics/isolation & purification
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Substance Withdrawal Syndrome/*genetics
EDAT- 2007/08/11 09:00
MHDA- 2007/11/14 09:00
CRDT- 2007/08/11 09:00
PHST- 2006/09/01 00:00 [received]
PHST- 2007/04/27 00:00 [revised]
PHST- 2007/04/28 00:00 [accepted]
PHST- 2007/08/11 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/08/11 09:00 [entrez]
AID - S0143-4179(07)00044-3 [pii]
AID - 10.1016/j.npep.2007.04.007 [doi]
PST - ppublish
SO  - Neuropeptides. 2007 Oct;41(5):321-8. doi: 10.1016/j.npep.2007.04.007. Epub 2007 
      Aug 3.