PMID- 17690108
OWN - NLM
STAT- MEDLINE
DCOM- 20071212
LR  - 20220409
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 282
IP  - 45
DP  - 2007 Nov 9
TI  - Parathyroid hormone stimulates osteoblastic expression of MCP-1 to recruit and 
      increase the fusion of pre/osteoclasts.
PG  - 33098-106
AB  - The clinical findings that alendronate blunted the anabolic effect of human 
      parathyroid hormone (PTH) on bone formation suggest that active resorption is 
      involved and enhances the anabolic effect. PTH signals via its receptor on the 
      osteoblast membrane, and osteoclasts are impacted indirectly via the products of 
      osteoblasts. Microarray with RNA from rats injected with human PTH or vehicle 
      showed a strong association between the stimulation of monocyte chemoattractant 
      protein-1 (MCP-1) and the anabolic effects of PTH. PTH rapidly and dramatically 
      stimulated MCP-1 mRNA in the femora of rats receiving daily injections of PTH or 
      in primary osteoblastic and UMR 106-01 cells. The stimulation of MCP-1 mRNA was 
      dose-dependent and a primary response to PTH signaling via the cAMP-dependent 
      protein kinase pathway in vitro. Studies with the mouse monocyte cell line RAW 
      264.7 and mouse bone marrow proved that osteoblastic MCP-1 can potently recruit 
      osteoclast monocyte precursors and facilitate receptor activator of NF-kappaB 
      ligand-induced osteoclastogenesis and, in particular, enhanced fusion. Our model 
      suggests that PTH-induced osteoblastic expression of MCP-1 is involved in 
      recruitment and differentiation at the stage of multinucleation of osteoclast 
      precursors. This information provides a rationale for increased osteoclast 
      activity in the anabolic effects of PTH in addition to receptor activator of 
      NF-kappaB ligand stimulation to initiate greater bone remodeling.
FAU - Li, Xin
AU  - Li X
AD  - Department of Physiology and Biophysics, University of Medicine and Dentistry of 
      New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 
      08854, USA.
FAU - Qin, Ling
AU  - Qin L
FAU - Bergenstock, Marika
AU  - Bergenstock M
FAU - Bevelock, Laura M
AU  - Bevelock LM
FAU - Novack, Deborah V
AU  - Novack DV
FAU - Partridge, Nicola C
AU  - Partridge NC
LA  - eng
GR  - DK 48109/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070809
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Parathyroid Hormone)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Fusion
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mice
MH  - Osteoblasts/cytology/*drug effects/*metabolism
MH  - Osteoclasts/cytology/*drug effects/*metabolism
MH  - Parathyroid Hormone/*pharmacology
MH  - RANK Ligand/pharmacology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Signal Transduction
EDAT- 2007/08/11 09:00
MHDA- 2007/12/13 09:00
CRDT- 2007/08/11 09:00
PHST- 2007/08/11 09:00 [pubmed]
PHST- 2007/12/13 09:00 [medline]
PHST- 2007/08/11 09:00 [entrez]
AID - S0021-9258(20)61319-9 [pii]
AID - 10.1074/jbc.M611781200 [doi]
PST - ppublish
SO  - J Biol Chem. 2007 Nov 9;282(45):33098-106. doi: 10.1074/jbc.M611781200. Epub 2007 
      Aug 9.