PMID- 17690217
OWN - NLM
STAT- MEDLINE
DCOM- 20071203
LR  - 20230513
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 9
IP  - 4
DP  - 2007 Sep
TI  - Detection of genetic alterations by immunoFISH analysis of whole cells extracted 
      from routine biopsy material.
PG  - 479-89
AB  - The detection of genetic abnormalities (eg, translocations, amplifications) in 
      paraffin-embedded samples by the fluorescence in situ hybridization (FISH) 
      technique is usually performed on tissue sections. FISH analysis of nuclei 
      extracted from paraffin-embedded samples is also possible, but the technique is 
      not widely used, principally because of the extra labor involved and the loss of 
      information on tissue architecture. In this article, we report that nuclei 
      extracted from paraffin-embedded tissue often retain at least part of the 
      surrounding cytoplasm. Consequently, immunocytochemical labeling for a range of 
      cellular markers (eg, of lineage or proliferation) can be performed in 
      combination with FISH labeling, allowing specific cell populations to be analyzed 
      for genetic abnormalities. These cell preparations are largely free of the 
      problems associated with tissue sections (eg, truncation artifact, signals in 
      different focal planes) so that interpretation is easy and numerical chromosomal 
      abnormalities are readily assessed. Cells isolated from paraffin sections can be 
      stored in suspension so that arrays can be created as and when needed from a 
      range of neoplasms for investigation by the immunoFISH technique (for example, 
      for studying a new genetic abnormality). This procedure represents a novel 
      methodology, which in some settings offers clear advantages over analysis of 
      tissue sections.
FAU - Mattsson, Goran
AU  - Mattsson G
AD  - Haematology Department, Level 4, John Radcliffe Hospital, Oxford OX3 9DU, UK.
FAU - Tan, Soo Yong
AU  - Tan SY
FAU - Ferguson, David J P
AU  - Ferguson DJ
FAU - Erber, Wendy
AU  - Erber W
FAU - Turner, Susan H
AU  - Turner SH
FAU - Marafioti, Teresa
AU  - Marafioti T
FAU - Mason, David Y
AU  - Mason DY
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070809
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (Cell Extracts)
SB  - IM
MH  - Biopsy
MH  - *Cell Extracts
MH  - Cell Separation
MH  - *Chromosome Aberrations
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Neoplasms/*diagnosis/*genetics/pathology
MH  - Palatine Tonsil/pathology/ultrastructure
MH  - Paraffin Embedding
MH  - Tissue Fixation
PMC - PMC1975102
EDAT- 2007/08/11 09:00
MHDA- 2007/12/06 09:00
PMCR- 2008/09/01
CRDT- 2007/08/11 09:00
PHST- 2007/08/11 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/11 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - S1525-1578(10)60420-5 [pii]
AID - 07-0041 [pii]
AID - 10.2353/jmoldx.2007.070041 [doi]
PST - ppublish
SO  - J Mol Diagn. 2007 Sep;9(4):479-89. doi: 10.2353/jmoldx.2007.070041. Epub 2007 Aug 
      9.