PMID- 17693184
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20081121
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 177
IP  - 1
DP  - 2007 Aug
TI  - Heterogeneity of PLAG1 gene rearrangements in pleomorphic adenoma.
PG  - 1-5
AB  - Pleomorphic adenoma (PA), a benign mixed salivary gland tumor, has been 
      associated with abnormal karyotypes in up to 70% of cases, with nonrandom 
      involvement of 8q12, the locus of the pleomorphic adenoma (PLAG1) gene. In this 
      study, cytogenetics and fluorescence in situ hybridization (FISH) were used to 
      investigate PLAG1 involvement in PA from seven patients. There were two males and 
      five females ranging in age from 25 to 65 years. Samples of parotid gland tissue 
      from the tumor sites, set up as solid tumor cultures, showed a normal karyotype 
      in two cases [46,XY;46,XX] and cytogenetic abnormalities in five cases (71%). The 
      abnormalities comprised one variant translocation [t(1;4;8)(p32;q35;q12)], two 
      classic translocations [t(5;8)(p13;q12)], one novel deletion 
      [del(12)(p11.2p12.1)], and a novel insertion [ins(9;8)(p22;q12q21.1)]. FISH was 
      performed in all cases by using two probes from the RP11 library, flanking PLAG1; 
      a sequence 1.48 megabases (Mb) upstream and another 2.27 Mb downstream, covering 
      a total area of 3.8 Mb. The PLAG1 gene was intact and normally situated in four 
      cases - the 46,XY, 46,XX, del(12p), and one t(5;8). PLAG1 was disrupted in three 
      cases - one t(5;8), ins(9;8), and t(1;4;8). In addition, genomic instability was 
      seen in two cases, one with PLAG1 amplification in the form of a homogeneously 
      staining region, and the other in der(8) ring formation. The data provide further 
      unique cases showing the complexity of PLAG1 gene rearrangements in PA.
FAU - Kandasamy, Jothy
AU  - Kandasamy J
AD  - Department of Cytogenetics, Children's Hospital at Westmead, Locked Bag 4001, 
      Westmead NSW 2145, Australia.
FAU - Smith, Arabella
AU  - Smith A
FAU - Diaz, Sara
AU  - Diaz S
FAU - Rose, Barbara
AU  - Rose B
FAU - O'Brien, Christopher
AU  - O'Brien C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (PLAG1 protein, human)
SB  - IM
MH  - Adenoma, Pleomorphic/*genetics
MH  - Adult
MH  - Aged
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Rearrangement
MH  - *Genetic Variation
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Salivary Gland Neoplasms/*genetics
MH  - Translocation, Genetic
EDAT- 2007/08/19 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/01/30 00:00 [received]
PHST- 2007/03/22 00:00 [revised]
PHST- 2007/04/18 00:00 [accepted]
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - S0165-4608(07)00220-8 [pii]
AID - 10.1016/j.cancergencyto.2007.04.006 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Aug;177(1):1-5. doi: 
      10.1016/j.cancergencyto.2007.04.006.