PMID- 17693318
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20141120
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 598
IP  - 1
DP  - 2007 Aug 13
TI  - Alpha-glucosidase based bismuth film electrode for inhibitor detection.
PG  - 143-6
AB  - A biosensing system based on alpha-glucosidase (AG) activity was developed by 
      using bismuth film modified glassy carbon electrode (BiFE). AG enzyme was 
      immobilized on the BiFE by means of gelatin membrane and the activity was 
      measured by the following of liberated 4-nitrophenol from the 
      4-nitrophenyl-alpha-D-glucopyranoside (PNPGP) which is the synthetic substrate of 
      the enzyme at the working potential of -950 mV. The proposed system was used as 
      an AG based biosensing system. Experimental data showed that the response current 
      of 4-nitrophenol obtained at the BiFE was linear in concentration range between 
      0.033 and 0.33 mM of PNPGP. Before examining the analytical characteristics, pH 
      optimization of the AG-biosensor was also performed. Furthermore, the proposed 
      method was applied to analyze two different AG inhibitors (Amaryl and Acorbose) 
      which are important in Noninsulin-dependent diabetes mellitus (NIDDM).
FAU - Timur, Suna
AU  - Timur S
AD  - Ege University, Faculty of Science, Biochemistry Department, Bornova 35100, 
      Izmir, Turkiye. suna.timur@ege.edu.tr
FAU - Anik, Ulku
AU  - Anik U
LA  - eng
PT  - Journal Article
DEP - 20070713
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Glycoside Hydrolase Inhibitors)
RN  - 0 (Nitrophenols)
RN  - 0 (Sulfonylurea Compounds)
RN  - 6KY687524K (glimepiride)
RN  - EC 3.2.1.20 (alpha-Glucosidases)
RN  - U015TT5I8H (Bismuth)
RN  - Y92ZL45L4R (4-nitrophenol)
SB  - IM
MH  - Biosensing Techniques
MH  - Bismuth/*chemistry
MH  - Electrochemistry
MH  - Electrodes
MH  - Enzyme Inhibitors/*analysis/pharmacology
MH  - Enzymes, Immobilized/*chemistry
MH  - Glycoside Hydrolase Inhibitors
MH  - Hydrogen-Ion Concentration
MH  - Nitrophenols/*chemistry
MH  - Sulfonylurea Compounds/analysis/pharmacology
MH  - alpha-Glucosidases/*chemistry/metabolism
EDAT- 2007/08/19 09:00
MHDA- 2007/09/29 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/03/22 00:00 [received]
PHST- 2007/07/02 00:00 [revised]
PHST- 2007/07/09 00:00 [accepted]
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - S0003-2670(07)01174-9 [pii]
AID - 10.1016/j.aca.2007.07.019 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2007 Aug 13;598(1):143-6. doi: 10.1016/j.aca.2007.07.019. Epub 
      2007 Jul 13.