PMID- 17696517
OWN - NLM
STAT- MEDLINE
DCOM- 20071108
LR  - 20070907
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 6
IP  - 9
DP  - 2007 Sep
TI  - Design of recombinant antibody microarrays for serum protein profiling: targeting 
      of complement proteins.
PG  - 3527-36
AB  - Antibody-based microarrays is a novel technology with great promise for 
      high-throughput proteomics. The process of designing high-performing arrays has, 
      however, turned out to be challenging. Here, we have designed the next generation 
      of a human recombinant scFv antibody microarray platform for protein expression 
      profiling of nonfractionated biotinylated human plasma and serum proteomes. The 
      setup, based on black polymer Maxisorb slides interfaced with a fluorescent-based 
      read-out system, was found to provide specific, sensitive (subpicomolar (pM) 
      range) and reproducible means for protein profiling. Further, a chip-to-chip 
      normalization protocol critical for comparing data generated on different chips 
      was devised. Finally, the microarray data were found to correlate well with 
      clinical laboratory data obtained using conventional methods, as demonstrated for 
      a set of medium abundant (micromolar (microM) to nanomolar (nM) range) protein 
      analytes in serum and plasma samples derived from healthy and 
      complement-deficient individuals.
FAU - Ingvarsson, Johan
AU  - Ingvarsson J
AD  - Department of Immunotechnology, BMC D13, Lund University, SE-221 84 Lund, Sweden.
FAU - Larsson, Anette
AU  - Larsson A
FAU - Sjoholm, Anders G
AU  - Sjoholm AG
FAU - Truedsson, Lennart
AU  - Truedsson L
FAU - Jansson, Bo
AU  - Jansson B
FAU - Borrebaeck, Carl A K
AU  - Borrebaeck CA
FAU - Wingren, Christer
AU  - Wingren C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070816
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Blood Proteins)
RN  - 0 (Proteins)
RN  - 0 (Proteome)
RN  - 0 (Recombinant Proteins)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Biotinylation
MH  - Blood Proteins/chemistry
MH  - Complement System Proteins/chemistry
MH  - Humans
MH  - Models, Chemical
MH  - Protein Array Analysis/*methods
MH  - Protein Transport
MH  - Proteins/chemistry
MH  - Proteome
MH  - Proteomics/*methods
MH  - Recombinant Proteins/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2007/08/19 09:00
MHDA- 2007/11/09 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/11/09 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - 10.1021/pr070204f [doi]
PST - ppublish
SO  - J Proteome Res. 2007 Sep;6(9):3527-36. doi: 10.1021/pr070204f. Epub 2007 Aug 16.