PMID- 17697430
OWN - NLM
STAT- MEDLINE
DCOM- 20080311
LR  - 20151119
IS  - 0007-1145 (Print)
IS  - 0007-1145 (Linking)
VI  - 99
IP  - 2
DP  - 2008 Feb
TI  - Responses of surrogate markers of cholesterol absorption and synthesis to changes 
      in cholesterol metabolism during various amounts of fat and cholesterol feeding 
      among healthy men.
PG  - 370-8
AB  - Serum ratios to cholesterol of lathosterol, and of cholestanol, campesterol and 
      sitosterol measure respective relative cholesterol synthesis and absorption, but 
      their clinical applicability is not known in evaluation of cholesterol metabolism 
      under different dietary conditions. We compared relative synthesis and absorption 
      of cholesterol to the respective absolute ones in healthy male volunteers (n 29) 
      on four subsequent diets: baseline home (HD), low-cholesterol low-fat (LCLF), 
      high-cholesterol low-fat (HCLF) and low-cholesterol high-fat (LCHF). Serum 
      lipids, lipoproteins, sterols, fractional cholesterol absorption and sterol 
      synthesis were examined. HCLF and LCHF decreased fractional cholesterol 
      absorption by approximately 23-27 % from baseline HD (P < 0.05) and increased the 
      levels of total and LDL-cholesterol in serum from LCLF by approximately 9-14 % (P 
      < 0.05). On HCLF, bile acid synthesis was high (P < 0.05 for each), and absolute 
      cholesterol synthesis tended to be higher than on HD and LCHF (NS). Relative 
      synthesis was positively associated with absolute cholesterol synthesis, but 
      inversely with relative absorption during each diet (P < 0.05). The relative 
      absorption markers were interrelated in each diet, and were also associated with 
      fractional absorption of cholesterol in each diet but HD. In conclusion, relative 
      markers of cholesterol absorption and synthesis reflect changes in cholesterol 
      metabolism despite the amount of dietary fat and cholesterol consumed, but their 
      validity with this respect is strengthened by controlled diets in metabolic 
      studies. Additions of cholesterol and fat to a diet low in fat and cholesterol 
      cause practically equal changes in the serum lipid profiles, whereas synthesis of 
      cholesterol (NS) and bile acids (P < 0.05) were higher with the high-cholesterol 
      feeding.
FAU - Nissinen, Markku J
AU  - Nissinen MJ
AD  - Department of Medicine, University of Helsinki, Helsinki, Finland. 
      markku.nissinen@hus.fi
FAU - Gylling, Helena
AU  - Gylling H
FAU - Miettinen, Tatu A
AU  - Miettinen TA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070815
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Biomarkers)
RN  - 0 (Cholesterol, Dietary)
RN  - 0 (Dietary Fats)
RN  - 0 (Lipids)
RN  - 0 (Lipoproteins)
RN  - 0 (Phytosterols)
RN  - 0 (Sitosterols)
RN  - 0 (Sterols)
RN  - 5L5O665639 (campesterol)
RN  - 5LI01C78DD (gamma-sitosterol)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Biomarkers/blood
MH  - Cholesterol/analogs & derivatives/biosynthesis/blood/*metabolism
MH  - Cholesterol, Dietary/administration & dosage/pharmacology
MH  - Diet
MH  - Dietary Fats/*administration & dosage/pharmacology
MH  - Feces/chemistry
MH  - Follow-Up Studies
MH  - Humans
MH  - *Intestinal Absorption/drug effects
MH  - Lipids/blood
MH  - Lipoproteins/blood
MH  - Male
MH  - Middle Aged
MH  - Phytosterols/blood/metabolism
MH  - Sitosterols/blood
MH  - Sterols/blood/metabolism
EDAT- 2007/08/19 09:00
MHDA- 2008/03/12 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2008/03/12 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - S0007114507811998 [pii]
AID - 10.1017/S0007114507811998 [doi]
PST - ppublish
SO  - Br J Nutr. 2008 Feb;99(2):370-8. doi: 10.1017/S0007114507811998. Epub 2007 Aug 
      15.