PMID- 17702266
OWN - NLM
STAT- MEDLINE
DCOM- 20070919
LR  - 20190513
IS  - 0161-8105 (Print)
IS  - 1550-9109 (Electronic)
IS  - 0161-8105 (Linking)
VI  - 30
IP  - 8
DP  - 2007 Aug
TI  - Identification of differentially expressed genes in blood cells of narcolepsy 
      patients.
PG  - 974-9
AB  - STUDY OBJECTIVE: A close association between the human leukocyte antigen 
      (HLA)-DRB1*1501/DQB1*0602 and abnormalities in some inflammatory cytokines have 
      been demonstrated in narcolepsy. Specific alterations in the immune system have 
      been suggested to occur in this disorder. We attempted to identify alterations in 
      gene expression underlying the abnormalities in the blood cells of narcoleptic 
      patients. DESIGNS: Total RNA from 12 narcolepsy-cataplexy patients and from 12 
      age- and sex-matched healthy controls were pooled. The pooled samples were 
      initially screened for candidate genes for narcolepsy by differential display 
      analysis using annealing control primers (ACP). The second screening of the 
      samples was carried out by semiquantitative PCR using gene-specific primers. 
      Finally, the expression levels of the candidate genes were further confirmed by 
      quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy 
      patients and 20 healthy controls). RESULTS: The second screening revealed 
      differential expression of 4 candidate genes. Among them, MX2 was confirmed as a 
      significantly down-regulated gene in the white blood cells of narcoleptic 
      patients by quantitative real-time PCR. CONCLUSION: We found the MX2 gene to be 
      significantly less expressed in comparison with normal subjects in the white 
      blood cells of narcoleptic patients. This gene is relevant to the immune system. 
      Although differential display analysis using ACP technology has a limitation in 
      that it does not help in determining the functional mechanism underlying 
      sleep/wakefulness dysregulation, it is useful for identifying novel genetic 
      factors related to narcolepsy, such as HLA molecules. Further studies are 
      required to explore the functional relationship between the MX2 gene and 
      narcolepsy pathophysiology.
FAU - Tanaka, Susumu
AU  - Tanaka S
AD  - The Sleep Disorders Project, Department of Sleep Disorders Research, Tokyo 
      Institute of Psychiatry, Setagaya-ku, Tokyo, Japan. stanaka@prit.go.jp
FAU - Honda, Yutaka
AU  - Honda Y
FAU - Honda, Makoto
AU  - Honda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Sleep
JT  - Sleep
JID - 7809084
RN  - 0 (Carrier Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*15:01 antigen)
RN  - 0 (IFI6 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Myxovirus Resistance Proteins)
RN  - 0 (PRPF8 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (beta 2-Microglobulin)
RN  - EC 3.6.1.- (ATP6V1E2 protein, human)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - Carrier Proteins/genetics
MH  - Cataplexy/*genetics/immunology
MH  - Down-Regulation/genetics
MH  - GTP-Binding Proteins/*genetics
MH  - Gene Expression/physiology
MH  - Genetic Markers/genetics
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Leukocytes/*metabolism
MH  - Membrane Glycoproteins/*genetics
MH  - Mitochondrial Proteins/genetics
MH  - Myxovirus Resistance Proteins
MH  - Narcolepsy/*genetics/immunology
MH  - RNA, Messenger/genetics
MH  - RNA-Binding Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vacuolar Proton-Translocating ATPases/genetics
MH  - beta 2-Microglobulin/genetics
PMC - PMC1978392
EDAT- 2007/08/19 09:00
MHDA- 2007/09/20 09:00
PMCR- 2008/02/01
CRDT- 2007/08/19 09:00
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/09/20 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - 10.1093/sleep/30.8.974 [doi]
PST - ppublish
SO  - Sleep. 2007 Aug;30(8):974-9. doi: 10.1093/sleep/30.8.974.