PMID- 17702615
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20240109
IS  - 1074-7613 (Print)
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 27
IP  - 2
DP  - 2007 Aug
TI  - The feedback phase of type I interferon induction in dendritic cells requires 
      interferon regulatory factor 8.
PG  - 228-39
AB  - Dendritic cells (DCs) produce type I interferons (IFNs) in greater amounts than 
      other cells, but the mechanisms remain elusive. Here we studied the role of a 
      transcription factor, IRF8, in DC induction of type I IFNs. Upon newcastle 
      disease virus (NDV) infection, bone marrow-derived plasmacytoid and conventional 
      DCs induced IFN transcripts, exhibiting two-phase kinetics. The second, 
      amplifying phase represented an IFN feedback response that accounted for much of 
      IFN protein production. Induction of second phase transcription required IRF8. 
      Mouse cytomegalovirus (MCMV) and Toll-like receptor-mediated IFN induction in DCs 
      also required IRF8. Chromatin immunoprecipitation analysis showed that IRF7, 
      IRF8, and RNA polymerase II were recruited to the IFN promoters upon stimulation. 
      Moreover, sustained RNA polymerase II recruitment to the promoters critically 
      depended on IRF8. Together, these data indicate that IRF8 magnifies the second 
      phase of IFN transcription in DCs by prolonging binding of basic transcription 
      machinery to the IFN promoters, thereby playing a role in innate immunity.
FAU - Tailor, Prafullakumar
AU  - Tailor P
AD  - Laboratory of Molecular Growth Regulation, National Institute of Child Health and 
      Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Tamura, Tomohiko
AU  - Tamura T
FAU - Kong, Hee Jeong
AU  - Kong HJ
FAU - Kubota, Toru
AU  - Kubota T
FAU - Kubota, Mayumi
AU  - Kubota M
FAU - Borghi, Paola
AU  - Borghi P
FAU - Gabriele, Lucia
AU  - Gabriele L
FAU - Ozato, Keiko
AU  - Ozato K
LA  - eng
GR  - Z01 HD001310-21/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20070816
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (Interferon Type I)
RN  - 0 (Irf7 protein, mouse)
RN  - 0 (interferon regulatory factor-8)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Chromatin Immunoprecipitation
MH  - Dendritic Cells/*immunology
MH  - Feedback, Physiological
MH  - Fibroblasts/immunology
MH  - *Gene Expression Regulation
MH  - Interferon Regulatory Factor-7/metabolism
MH  - Interferon Regulatory Factors/genetics/*metabolism
MH  - Interferon Type I/*genetics
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Promoter Regions, Genetic
MH  - RNA Polymerase II/metabolism
PMC - PMC2768351
MID - NIHMS30684
EDAT- 2007/08/19 09:00
MHDA- 2007/11/14 09:00
PMCR- 2009/10/27
CRDT- 2007/08/19 09:00
PHST- 2006/09/19 00:00 [received]
PHST- 2007/04/03 00:00 [revised]
PHST- 2007/06/11 00:00 [accepted]
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
PHST- 2009/10/27 00:00 [pmc-release]
AID - S1074-7613(07)00368-8 [pii]
AID - 10.1016/j.immuni.2007.06.009 [doi]
PST - ppublish
SO  - Immunity. 2007 Aug;27(2):228-39. doi: 10.1016/j.immuni.2007.06.009. Epub 2007 Aug 
      16.