PMID- 17702974
OWN - NLM
STAT- MEDLINE
DCOM- 20071212
LR  - 20071214
IS  - 0022-1503 (Print)
IS  - 0022-1503 (Linking)
VI  - 98
IP  - 5
DP  - 2007
TI  - A cytogenetically characterized, genome-anchored 10-Mb BAC set and CGH array for 
      the domestic dog.
PG  - 474-84
AB  - The generation of a 7.5x dog genome assembly provides exciting new opportunities 
      to interpret tumor-associated chromosome aberrations at the biological level. We 
      present a genomic microarray for array comparative genomic hybridization (aCGH) 
      analysis in the dog, comprising 275 bacterial artificial chromosome (BAC) clones 
      spaced at intervals of approximately 10 Mb. Each clone has been positioned 
      accurately within the genome assembly and assigned to a unique chromosome 
      location by fluorescence in situ hybridization (FISH) analysis, both individually 
      and as chromosome-specific BAC pools. The microarray also contains clones 
      representing the dog orthologues of 31 genes implicated in human cancers. FISH 
      analysis of the 10-Mb BAC clone set indicated excellent coverage of each dog 
      chromosome by the genome assembly. The order of clones was consistent with the 
      assembly, but the cytogenetic intervals between clones were variable. We 
      demonstrate the application of the BAC array for aCGH analysis to identify both 
      whole and partial chromosome imbalances using a canine histiocytic sarcoma case. 
      Using BAC clones selected from the array as probes, multicolor FISH analysis was 
      used to further characterize these imbalances, revealing numerous structural 
      chromosome rearrangements. We outline the value of a combined aCGH/FISH approach, 
      together with a well-annotated dog genome assembly, in canine and comparative 
      cancer studies.
FAU - Thomas, Rachael
AU  - Thomas R
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, 
      USA.
FAU - Duke, Shannon E
AU  - Duke SE
FAU - Bloom, Stephanie K
AU  - Bloom SK
FAU - Breen, Tessa E
AU  - Breen TE
FAU - Young, Andrea C
AU  - Young AC
FAU - Feiste, Erika
AU  - Feiste E
FAU - Seiser, Eric L
AU  - Seiser EL
FAU - Tsai, Pei-Chien
AU  - Tsai PC
FAU - Langford, Cordelia F
AU  - Langford CF
FAU - Ellis, Peter
AU  - Ellis P
FAU - Karlsson, Elinor K
AU  - Karlsson EK
FAU - Lindblad-Toh, Kerstin
AU  - Lindblad-Toh K
FAU - Breen, Matthew
AU  - Breen M
LA  - eng
GR  - R21 NS051190-01/NS/NINDS NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070816
PL  - United States
TA  - J Hered
JT  - The Journal of heredity
JID - 0375373
SB  - IM
EIN - J Hered. 2007 Sep-Oct;98(6):635-6
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Dogs/*genetics
MH  - Genome
MH  - In Situ Hybridization, Fluorescence
MH  - *Nucleic Acid Hybridization
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Sensitivity and Specificity
EDAT- 2007/08/19 09:00
MHDA- 2007/12/13 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/12/13 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - esm053 [pii]
AID - 10.1093/jhered/esm053 [doi]
PST - ppublish
SO  - J Hered. 2007;98(5):474-84. doi: 10.1093/jhered/esm053. Epub 2007 Aug 16.