PMID- 17703298
OWN - NLM
STAT- MEDLINE
DCOM- 20071127
LR  - 20131121
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 76
IP  - 5
DP  - 2007 Oct
TI  - Quantification of anammox populations enriched in an immobilized microbial 
      consortium with low levels of ammonium nitrogen and at low temperature.
PG  - 1173-9
AB  - Anaerobic ammonium oxidizing (anammox) bacteria present in microbial communities 
      in two laboratory-scale upflow anoxic reactors supplied with small amounts of 
      ammonium (<3 mg/l) at low temperature were detected and quantified. The reactors, 
      operated at 20 degrees C, were seeded with an immobilized microbial consortium 
      (IMC) and anaerobic granules (AG) from an upflow anaerobic sludge blanket (UASB) 
      treating brewery wastewater. Our results showed that complete ammonium and 
      nitrite removal with greater than 92% total nitrogen removal efficiency was 
      achieved in the reactor inoculated with both the IMC and AG, while that of the 
      reactor inoculated with only the IMC was lower than 40%; enrichment was 
      successful after the addition of AG. Quantitative fluorescence in situ 
      hybridization (FISH) analysis confirmed that anammox bacteria were present only 
      in the reactor inoculated with an IMC and AG. The copy number of the 16S-rRNA 
      gene of the anammox bacteria calculated by most probable number-polymerase chain 
      reaction (MPN-PCR) from the total DNA extracted from both reactors (2.5 x 10(4) 
      copies/mug of DNA) was two orders lower than that of the domain bacteria (2.5 x 
      10(6) copies/mug of DNA). The results revealed that immobilized multiple seed 
      sludges were optimal for anammox enrichment at low temperature and ammonium 
      concentrations.
FAU - Pathak, Bipin K
AU  - Pathak BK
AD  - Department of Environmental Science and Engineering, Kathmandu University, P.O. 
      Box 6250, Kavre, Nepal. pathakbipin@gmail.com
FAU - Kazama, Futaba
AU  - Kazama F
FAU - Tanaka, Yasuhiro
AU  - Tanaka Y
FAU - Mori, Kazuhiro
AU  - Mori K
FAU - Sumino, Tatsuo
AU  - Sumino T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070817
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (DNA, Bacterial)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (Sewage)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria, Anaerobic/classification/*genetics/*growth & development/metabolism
MH  - Bioreactors
MH  - *Cells, Immobilized
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - *Ecosystem
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen/*metabolism
MH  - Polymerase Chain Reaction
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - Sewage/microbiology
MH  - Temperature
MH  - Waste Disposal, Fluid
EDAT- 2007/08/19 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/19 09:00
PHST- 2007/02/22 00:00 [received]
PHST- 2007/05/02 00:00 [accepted]
PHST- 2007/04/30 00:00 [revised]
PHST- 2007/08/19 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/19 09:00 [entrez]
AID - 10.1007/s00253-007-1026-5 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2007 Oct;76(5):1173-9. doi: 10.1007/s00253-007-1026-5. 
      Epub 2007 Aug 17.