PMID- 17704012
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20131121
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 857
IP  - 2
DP  - 2007 Oct 1
TI  - Determination of serum uric acid using high-performance liquid chromatography 
      (HPLC)/isotope dilution mass spectrometry (ID-MS) as a candidate reference 
      method.
PG  - 287-95
AB  - Uric acid is an important diagnostic marker of catabolism of the purine 
      nucleosides, and accurate measurements of serum uric acid are necessary for 
      proper diagnosis of gout or renal disease appearance. A candidate reference 
      method involving isotope dilution coupled with liquid chromatography/mass 
      spectrometry (LC/MS) has been described. An isotopically labeled internal 
      standard, [1,3-(15)N(2)] uric acid, was added to serum, followed by equilibration 
      and protein removal clean up to prepare samples for liquid chromatography/mass 
      spectrometry electrospray ionization (LC/MS-ESI) analyses. (M-H)(-) ions at m/z 
      167 and 169 for uric acid and its labeled internal standard were monitored for 
      LC/MS. The accuracy of the measurement was evaluated by a comparison of results 
      of this candidate reference method on lyophilized human serum reference materials 
      for uric acid (Standard Reference Materials SRM909b) with the certified values 
      determined by gas chromatography/mass spectrometry reference methods and by a 
      recovery study for the added uric acid. The method performed well against the 
      established reference method of ion-exchange followed by derivatization isotope 
      dilution (ID) gas chromatography mass spectrometry (ID-GC/MS). The results of 
      this method for uric acid agreed well with the certified values and were within 
      0.10%. The amounts of uric acid recovered and added were in good agreement for 
      the three concentrations. This method was applied to determine uric acid in 
      samples of frozen serum pools. Excellent precision was obtained with within-set 
      CVs of 0.08-0.18% and between-set CVs of 0.02-0.07% for LC/MS analyses. Liquid 
      chromatography/tandem mass spectrometry electrospray ionization (LC/MS/MS-ESI) 
      analysis was also performed. The LC/MS and LC/MS/MS results were in very good 
      agreement (within 0.14%). This LC/MS method, which demonstrates good accuracy and 
      precision, and is in the speed of analysis without the need for a derivatization 
      stage, qualifies as a candidate reference method. This method can be used as an 
      alternative reference method to provide an accuracy base to which the routine 
      methods can be compared.
FAU - Dai, Xinhua
AU  - Dai X
AD  - National Institute of Metrology, National Research Center for CRMs, Beijing 
      100013, China. xhdai.75@iccas.ac.cn
FAU - Fang, Xiang
AU  - Fang X
FAU - Zhang, Chunmei
AU  - Zhang C
FAU - Xu, Ruifeng
AU  - Xu R
FAU - Xu, Bei
AU  - Xu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070802
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Solutions)
RN  - 268B43MJ25 (Uric Acid)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Cryopreservation
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Reference Standards
MH  - Solutions
MH  - Uric Acid/*blood
EDAT- 2007/08/21 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/08/21 09:00
PHST- 2007/02/06 00:00 [received]
PHST- 2007/07/18 00:00 [revised]
PHST- 2007/07/23 00:00 [accepted]
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
AID - S1570-0232(07)00545-4 [pii]
AID - 10.1016/j.jchromb.2007.07.035 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 1;857(2):287-95. doi: 
      10.1016/j.jchromb.2007.07.035. Epub 2007 Aug 2.