PMID- 17704743
OWN - NLM
STAT- MEDLINE
DCOM- 20070928
LR  - 20100115
IS  - 1812-9269 (Print)
IS  - 1812-9269 (Linking)
VI  - 29
IP  - 2
DP  - 2007 Jun
TI  - Molecular cytogenetic aberrations in patients with multiple myeloma studied by 
      interphase fluorescence in situ hybridization.
PG  - 116-20
AB  - BACKGROUND: Multiple myeloma (MM) is an incurable hematological disorder 
      characterized by the accumulation of malignant plasma cells within the bone 
      marrow (BM). The clinical heterogeneity of MM is dictated by the cytogenetic 
      aberrations present in the clonal plasma cells (PCs). Cytogenetic studies in MM 
      are hampered by the hypoproliferative nature of plasma cells in MM. Therefore, 
      fluorescence in situ hybridization (FISH) analysis combined with 
      magnetic-activated cell sorting (MACS) is an attractive alternative for 
      evaluation of numerical and structural chromosomal changes in MM. METHODS: 
      Interphase FISH studies with three different specific probes for the regions 
      containing 13q14.3(D13S319), 14q32(IGHC/IGHV) and 1q12(CEP1) were performed in 48 
      MM patients. Interphase FISH studies with LSI IGH/CCND1, LSI IGH/FGFR3, and LSI 
      IGH/MAF probes were used to detect t(11;14)(q13;q32), t(4;14)(p16;q32), and 
      t(14;16)(q32;q23) in patients with 14q32 rearrangement. RESULTS: Molecular 
      cytogenetic aberrations were found in 40 (83.3%) of the 48 MM patients. 13 
      patients (27.1%) simultaneously had 13q deletion/monosomy 13[del(13q14)], 
      illegitimate IGH rearrangement and chromosome 1 abnormality. Del (13q14) was 
      detected in 21 cases (43.7%), and illegitimate IGH rearrangements in 29 (60.4%) 
      including 6 with t (11;14) and 5 with t(4;14). None of 9 patients with 
      illegitimate IGH rearrangements and without t(11;14) or t(4;14) we detected had 
      t(14;16)(q32;q23). 24 of the 48 MM patients (50%) had chromosome 1 abnormalities. 
      Among 21 patients with del (13q14), 15 patients had Amp1q12;16 had IgH 
      rearrangements. Whereas, among 27 cases without del (13q14), 8 had Amp1q12; 13 
      had IgH rearrangements. There was a strong association between del(13q14) and 
      Amp1q12 ( = 8.26, p < 0.01), and between del (13q14) and IgH rearrangement ( = 
      3.88, p < 0.05). CONCLUSION: 13q deletion/monosomy 13, IGH rearrangement and 
      chromosome 1 abnormality are frequent in MM. They are not randomly distributed, 
      but strongly interconnected. Interphase FISH technique combined with MACS using 
      CD138-specific antibody is a highly sensitive technique at detecting molecular 
      cytogenetic aberrations in MM.
FAU - Chen, L- J
AU  - Chen L
AD  - Department of Hematology, First Affiliated Hospital of Nanjing Medical 
      University, Jiangsu Province Hospital Nanjing 210029, China.
FAU - Li, J- Y
AU  - Li J
FAU - Xu, W
AU  - Xu W
FAU - Qiu, H- R
AU  - Qiu H
FAU - Zhu, Y
AU  - Zhu Y
FAU - Zhang, Y- P
AU  - Zhang Y
FAU - Duan, L- M
AU  - Duan L
FAU - Qian, S- X
AU  - Qian S
FAU - Lu, H
AU  - Lu H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ukraine
TA  - Exp Oncol
JT  - Experimental oncology
JID - 101230541
RN  - 0 (Syndecan-1)
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 14
MH  - Cytogenetic Analysis
MH  - Female
MH  - Flow Cytometry
MH  - Gene Deletion
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/*pathology
MH  - Neoplasm Staging
MH  - Syndecan-1/metabolism
EDAT- 2007/08/21 09:00
MHDA- 2007/09/29 09:00
CRDT- 2007/08/21 09:00
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2007/09/29 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
AID - 31/613 [pii]
PST - ppublish
SO  - Exp Oncol. 2007 Jun;29(2):116-20.