PMID- 17706207
OWN - NLM
STAT- MEDLINE
DCOM- 20080715
LR  - 20231213
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 89
IP  - 5 Suppl
DP  - 2008 May
TI  - Increased frequency of human leukocyte antigen-E inhibitory receptor 
      CD94/NKG2A-expressing peritoneal natural killer cells in patients with 
      endometriosis.
PG  - 1490-6
AB  - OBJECTIVE: To analyze the frequency of peritoneal natural killer (NK) cells 
      expressing the human leukocyte antigen (HLA)-E receptor CD94/NKG2A in patients 
      with endometriosis. DESIGN: Case-control study. SETTING: University hospital. 
      PATIENT(S): Stage III and stage IV endometriosis, according to the revised 
      American Society for Reproductive Medicine classification, was laparoscopically 
      and histologically confirmed in 11 and 9 patients, respectively; 13 subjects 
      without endometriosis were selected for the control group. INTERVENTION(S): 
      Collection of peripheral venous blood, peritoneal fluid, endometriotic tissue, 
      and normal endometrium in subjects undergoing laparoscopy. MAIN OUTCOME 
      MEASURE(S): Surface expression levels of CD94/NKG2A and CD94/NKG2C were detected 
      by three-color cytofluorometric analysis. Semiquantitative HLA-E messenger RNA 
      expression analysis was performed in endometriotic lesions and in eutopic 
      endometrium. NK cell-mediated cytotoxic activity toward HLA-E positive target, 
      DT360 cell line, was also determined. RESULT(S): In women with endometriosis, the 
      percentage of CD94/NKG2A-positive peritoneal NK cells was significantly higher 
      than in the control group. The CD94/NKG2A ligand, HLA-E, was detected at high 
      levels in endometriotic tissue as messenger RNA transcript. Target cells bearing 
      HLA-E were resistant to NK cell-mediated lysis in a CD94/NKG2A-dependent manner. 
      CONCLUSION(S): Increased expression of CD94/NKG2A in peritoneal NK cells may 
      mediate the resistance of endometriotic tissue to NK cell-mediated lysis, thus 
      contributing to the progression of the disease.
FAU - Galandrini, Ricciarda
AU  - Galandrini R
AD  - Department of Experimental Medicine, University "La Sapienza," Rome, Italy. 
      ricciarda.galandrini@uniroma1.it
FAU - Porpora, Maria Grazia
AU  - Porpora MG
FAU - Stoppacciaro, Antonella
AU  - Stoppacciaro A
FAU - Micucci, Federica
AU  - Micucci F
FAU - Capuano, Cristina
AU  - Capuano C
FAU - Tassi, Ilaria
AU  - Tassi I
FAU - Di Felice, Alessia
AU  - Di Felice A
FAU - Benedetti-Panici, Pierluigi
AU  - Benedetti-Panici P
FAU - Santoni, Angela
AU  - Santoni A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070813
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (KLRC2 protein, human)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily D)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Natural Killer Cell)
SB  - IM
MH  - Adult
MH  - Ascitic Fluid/*immunology/metabolism/pathology
MH  - Case-Control Studies
MH  - Disease Progression
MH  - Endometriosis/blood/*immunology/metabolism/pathology
MH  - Female
MH  - HLA Antigens/genetics/metabolism
MH  - Histocompatibility Antigens Class I/genetics/metabolism
MH  - Humans
MH  - Immune Tolerance/immunology
MH  - Immunity, Cellular/immunology
MH  - Killer Cells, Natural/metabolism/*pathology
MH  - Lymphocyte Count
MH  - Middle Aged
MH  - NK Cell Lectin-Like Receptor Subfamily C
MH  - NK Cell Lectin-Like Receptor Subfamily D/blood/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Immunologic/blood/*metabolism
MH  - Receptors, Natural Killer Cell
MH  - HLA-E Antigens
EDAT- 2007/08/21 09:00
MHDA- 2008/07/17 09:00
CRDT- 2007/08/21 09:00
PHST- 2006/11/22 00:00 [received]
PHST- 2007/05/04 00:00 [revised]
PHST- 2007/05/04 00:00 [accepted]
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
AID - S0015-0282(07)01143-0 [pii]
AID - 10.1016/j.fertnstert.2007.05.018 [doi]
PST - ppublish
SO  - Fertil Steril. 2008 May;89(5 Suppl):1490-6. doi: 
      10.1016/j.fertnstert.2007.05.018. Epub 2007 Aug 13.