PMID- 17706795
OWN - NLM
STAT- MEDLINE
DCOM- 20071128
LR  - 20171116
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 189
IP  - 1-2
DP  - 2007 Sep
TI  - A role for the neurotrophin receptor TrkB on maturing dendritic cells.
PG  - 88-94
AB  - BACKGROUND: Neurotrophins are involved in neuroimmune interactions. However, the 
      expression and role of neurotrophin receptors on dendritic cells have not been 
      systematically studied. METHODS: The neurotrophin receptors p75NTR, TrkA, TrkB 
      and TrkC were analyzed on immature and mature Human Monocyte-derived Dendritic 
      Cells (HMDCs) using flow cytometry. In addition, we compared the impact of five 
      different maturing protocols on the expression of neurotrophin receptors on 
      HMDCs. Finally, the effect of neurotrophins on surface molecule expression and 
      the survival of HMDCs was investigated. RESULTS: There was a low expression of 
      p75NTR, TrkA and TrkB on immature HMDCs. HMDCs at different maturation stages did 
      not show a significantly different expression of TrkA, as compared to immature 
      HMDCs. By contrast, there was an upregulation of TrkB on suboptimally, but not 
      optimally matured HMDC. In addition, there was a non-significant trend to a 
      parallel increase of p75NTR expression on these cells. Functional experiments 
      with maturing HMDCs revealed that both ligands of the TrkB receptor, 
      Brain-derived neurotrophic factor (BDNF) and Neurotrophin 4 (NT-4), significantly 
      increased the expression of markers of antigen presentation (HLA-DR and CD80) and 
      the survival of maturing HMDCs. CONCLUSION: These data suggest that the TrkB 
      ligands BDNF and NT-4 can directly influence human dendritic cells during their 
      maturation.
FAU - Bratke, Kai
AU  - Bratke K
AD  - Department of Pneumology, University of Rostock, Germany.
FAU - Maruschke, Lars
AU  - Maruschke L
FAU - Darowski, Martin
AU  - Darowski M
FAU - Kuepper, Michael
AU  - Kuepper M
FAU - Braun, Armin
AU  - Braun A
FAU - Virchow, J Christian
AU  - Virchow JC
FAU - Lommatzsch, Marek
AU  - Lommatzsch M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070815
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (B7-1 Antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Nerve Growth Factors)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - B7-1 Antigen/genetics/metabolism
MH  - Dendritic Cells/drug effects/*physiology
MH  - Flow Cytometry/methods
MH  - Gene Expression Regulation, Developmental/drug effects/physiology
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Nerve Growth Factors/pharmacology
MH  - Receptor, trkB/*physiology
EDAT- 2007/08/21 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/21 09:00
PHST- 2007/04/25 00:00 [received]
PHST- 2007/07/01 00:00 [revised]
PHST- 2007/07/11 00:00 [accepted]
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
AID - S0165-5728(07)00230-5 [pii]
AID - 10.1016/j.jneuroim.2007.07.013 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2007 Sep;189(1-2):88-94. doi: 10.1016/j.jneuroim.2007.07.013. 
      Epub 2007 Aug 15.