PMID- 17707700
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20070928
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 857
IP  - 2
DP  - 2007 Oct 1
TI  - Improved RP-HPLC method to determine neferine in dog plasma and its application 
      to pharmacokinetics.
PG  - 341-6
AB  - Existing methods to determine neferine, a bisbenzylisoquinline alkaloid, either 
      have no internal standard or lack selectivity, or take longer time. Here an 
      improved reverse-phase high-performance liquid chromatographic (RP-HPLC) method 
      was established in biological samples. The extraction recovery was 90.9% for 
      neferine at concentration level of 0.2 microg/ml and 77.7% for dauricine (the 
      internal standard) at 5 microg/ml in dog plasma, respectively. The linear 
      quantification range of the method was 25-2000 ng/ml in dog plasma, with linear 
      correlation coefficients greater than 0.999. The intra-day and inter-day relative 
      standard deviations (R.S.D.s) for neferine at 50, 200 and 1000 ng/ml levels in 
      dog plasma fell in the range of 3.0-5.4% and 4.3-9.5%, respectively. The RP-HPLC 
      method was successfully applied to a pharmacokinetics study, in which 
      experimental dogs received a single dose of neferine (5 mg/kg i.v. or 10 mg/kg 
      p.o.). The pharmacokinetic result was presented.
FAU - Zhao, Libo
AU  - Zhao L
AD  - Department of Pharmacology, Tongji Medical College, Huazhong University of 
      Science and Technology, China.
FAU - Wang, Xiaomin
AU  - Wang X
FAU - Wu, Jianhong
AU  - Wu J
FAU - Jia, Yongsheng
AU  - Jia Y
FAU - Wang, Wenquan
AU  - Wang W
FAU - Zhang, Shaohui
AU  - Zhang S
FAU - Wang, Jialing
AU  - Wang J
LA  - eng
PT  - Journal Article
DEP - 20070803
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Benzylisoquinolines)
RN  - 2292-16-2 (neferine)
SB  - IM
MH  - Animals
MH  - Benzylisoquinolines/*blood/chemistry/*pharmacokinetics
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dogs
MH  - Drug Stability
MH  - Male
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2007/08/21 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/08/21 09:00
PHST- 2007/05/14 00:00 [received]
PHST- 2007/07/24 00:00 [revised]
PHST- 2007/07/25 00:00 [accepted]
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
AID - S1570-0232(07)00553-3 [pii]
AID - 10.1016/j.jchromb.2007.07.043 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 1;857(2):341-6. doi: 
      10.1016/j.jchromb.2007.07.043. Epub 2007 Aug 3.