PMID- 17707923
OWN - NLM
STAT- MEDLINE
DCOM- 20071127
LR  - 20181113
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1769
IP  - 9-10
DP  - 2007 Sep-Oct
TI  - HDAC1 bound to the Cyp1a1 promoter blocks histone acetylation associated with Ah 
      receptor-mediated trans-activation.
PG  - 569-78
AB  - Metabolic bioactivation of polycyclic aromatic hydrocarbons, such as the 
      environmental procarcinogen benzo[a]pyrene, is catalyzed by a cytochrome P450 
      monooxygenase encoded by the substrate-inducible Cyp1a1 gene. Cyp1a1 induction 
      requires trans-activation by the heterodimeric transcriptional complex formed by 
      the liganded Ah receptor (AHR) and its partner, ARNT. Previously, we showed that 
      constitutively bound HDAC1 dissociates from Cyp1a1 promoter chromatin after 
      ligand-mediated induction, concomitantly with the recruitment of AHR/ARNT 
      complexes and p300. Here, we investigated the hypothesis that HDAC1 binding 
      maintains the Cyp1a1 gene in a silenced state in uninduced cells. We find that 
      Cyp1a1 induction by the AHR/ARNT is associated with modification of specific 
      chromatin marks, including hyperacetylation of histone H3K14 and H4K16, 
      trimethylation of histone H3K4, and phosphorylation of H3S10. HDAC1 and DNMT1 
      form complexes on the Cyp1a1 promoter of uninduced cells but HDAC1 inhibition 
      alone is not sufficient to induce Cyp1a1 expression, although it allows for the 
      hyperacetylation of H3K14 and H4K16 to levels similar to those found in 
      B[a]P-induced cells. These results show that by blocking the modification of 
      histone marks, HDAC1 plays a central role in Cyp1a1 expression and that its 
      removal is a necessary but not sufficient condition for Cyp1a1 induction, 
      underscoring the requirement for a concerted series of chromatin-remodeling 
      events to complete the initial steps of gene trans-activation by the Ah receptor.
FAU - Schnekenburger, Michael
AU  - Schnekenburger M
AD  - Center for Environmental Genetics and Department of Environmental Health, 
      University of Cincinnati Medical Center, P.O. Box 670056, Cincinnati, Ohio 
      45267-0056, USA.
FAU - Peng, Li
AU  - Peng L
FAU - Puga, Alvaro
AU  - Puga A
LA  - eng
GR  - R01 ES10807/ES/NIEHS NIH HHS/United States
GR  - R01 ES010807/ES/NIEHS NIH HHS/United States
GR  - R01 ES006273/ES/NIEHS NIH HHS/United States
GR  - P42 ES04908/ES/NIEHS NIH HHS/United States
GR  - P30 ES006096-14/ES/NIEHS NIH HHS/United States
GR  - P42 ES004908/ES/NIEHS NIH HHS/United States
GR  - P30 ES006096/ES/NIEHS NIH HHS/United States
GR  - P42 ES004908-130012/ES/NIEHS NIH HHS/United States
GR  - R01 ES06273/ES/NIEHS NIH HHS/United States
GR  - R01 ES010807-07/ES/NIEHS NIH HHS/United States
GR  - R01 ES006273-13/ES/NIEHS NIH HHS/United States
GR  - P30 ES06096/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070720
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Carrier Proteins)
RN  - 0 (Fetal Proteins)
RN  - 0 (Histones)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (TACC3 protein, mouse)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (Dnmt1 protein, mouse)
RN  - EC 2.3.1.48 (p300-CBP Transcription Factors)
RN  - EC 3.5.1.98 (Hdac1 protein, mouse)
RN  - EC 3.5.1.98 (Histone Deacetylase 1)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Benzo(a)pyrene/metabolism
MH  - Carrier Proteins/genetics/metabolism
MH  - Catalysis
MH  - Cell Line, Tumor
MH  - Chromatin Assembly and Disassembly/physiology
MH  - Cytochrome P-450 CYP1A1/*biosynthesis
MH  - DNA (Cytosine-5-)-Methyltransferase 1
MH  - DNA (Cytosine-5-)-Methyltransferases/metabolism
MH  - Enzyme Induction/physiology
MH  - Enzyme Repression/physiology
MH  - Fetal Proteins/genetics/metabolism
MH  - Histone Deacetylase 1
MH  - Histone Deacetylases/*metabolism
MH  - Histones/*metabolism
MH  - Methylation
MH  - Mice
MH  - Microtubule-Associated Proteins
MH  - Phosphorylation
MH  - Promoter Regions, Genetic/*physiology
MH  - Protein Binding/physiology
MH  - Protein Processing, Post-Translational/physiology
MH  - Receptors, Aryl Hydrocarbon/*metabolism
MH  - Transcriptional Activation/*physiology
MH  - p300-CBP Transcription Factors
PMC - PMC2083643
MID - NIHMS31273
EDAT- 2007/08/21 09:00
MHDA- 2007/12/06 09:00
PMCR- 2008/09/01
CRDT- 2007/08/21 09:00
PHST- 2007/05/25 00:00 [received]
PHST- 2007/07/12 00:00 [revised]
PHST- 2007/07/12 00:00 [accepted]
PHST- 2007/08/21 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/21 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - S0167-4781(07)00123-6 [pii]
AID - 10.1016/j.bbaexp.2007.07.002 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2007 Sep-Oct;1769(9-10):569-78. doi: 
      10.1016/j.bbaexp.2007.07.002. Epub 2007 Jul 20.