PMID- 17709401
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20211203
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 82
IP  - 5
DP  - 2007 Nov
TI  - Apoptosis triggered by phagocytosis-related oxidative stress through FLIPS 
      down-regulation and JNK activation.
PG  - 1344-52
AB  - Tumor necrosis factor-alpha (TNF-alpha)-activated neutrophils phagocytose and 
      eliminate bacteria by using such oxidants as hydrogen peroxide (H(2)O(2)) and 
      hypochlorous acid (HOCl), which is produced from H(2)O(2) by myeloperoxidase 
      (MPO). Thereafter, neutrophils eventually undergo apoptosis to prevent excessive 
      inflammation. However, it is unclear how this process is regulated. Here, we show 
      that cotreatment of TNF-alpha-resistant neutrophilic HL-60 cells with taurine 
      chloramine (TauCl), a detoxified form of HOCl, and TNF-alpha renders them 
      susceptible to apoptosis, mostly by preventing nuclear factor-kappaB (NF-kappaB) 
      activation. Of several NF-kappaB target genes tested, FLICE inhibitory protein 
      short form (FLIP(S)) was specifically down-regulated by TauCl. TNF-alpha/TauCl 
      cotreatment-induced apoptosis was largely blocked by stable expression of 
      FLIP(S). Cotreatment with TNF-alpha and H(2)O(2) promoted apoptotic signaling via 
      MPO activation and subsequent attenuation of FLIP(S) expression. TNF-alpha 
      priming with H(2)O(2) or bacteria caused MPO-dependent apoptosis in human 
      neutrophils. However, FLIP(S) knock-down by siRNA did not affect the viability of 
      cells treated with TNF-alpha, implying that TauCl may affect another pathway in 
      TNF-alpha-driven apoptosis. Indeed, oxidization of thioredoxin-1 (Trx-1) by TauCl 
      induced the activation of apoptosis signal-regulating kinase 1 (ASK1) and cJun 
      N-terminal kinase (JNK), thereby triggering TNF-alpha-mediated apoptosis. Taken 
      together, these results indicate that the antiapoptotic signaling induced by 
      TNF-alpha via NF-kappaB activation can be altered to promote apoptosis via 
      H(2)O(2)-MPO-mediated FLIP(S) down-regulation and JNK activation.
FAU - Kanayama, Atsuhiro
AU  - Kanayama A
AD  - Department of Integrated Biosciences, Graduate School of Frontier Sciences, 
      University of Tokyo, Bioscience Building 402, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 
      Japan. kanayama-atsuhiro@k.u-tokyo.ac.jp
FAU - Miyamoto, Yusei
AU  - Miyamoto Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070820
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antiviral Agents)
RN  - 0 (CASP8 and FADD-Like Apoptosis Regulating Protein)
RN  - 0 (CFLAR protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1EQV5MLY3D (Taurine)
RN  - 51036-13-6 (N-chlorotaurine)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 5)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Antiviral Agents/pharmacology
MH  - Apoptosis/*physiology
MH  - Blotting, Western
MH  - CASP8 and FADD-Like Apoptosis Regulating Protein/*metabolism
MH  - Caspases/metabolism
MH  - Cell Survival/drug effects
MH  - Down-Regulation
MH  - Enzyme Activation
MH  - Gene Expression Regulation, Neoplastic
MH  - HL-60 Cells
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - Kidney/cytology/metabolism
MH  - MAP Kinase Kinase Kinase 5/metabolism
MH  - NF-kappa B/metabolism
MH  - Neutrophils/drug effects/metabolism
MH  - *Oxidative Stress
MH  - Peroxidase/metabolism
MH  - *Phagocytosis
MH  - Protein Serine-Threonine Kinases/antagonists & inhibitors
MH  - Taurine/analogs & derivatives/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2007/08/22 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/08/22 09:00
PHST- 2007/08/22 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/08/22 09:00 [entrez]
AID - jlb.0407259 [pii]
AID - 10.1189/jlb.0407259 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2007 Nov;82(5):1344-52. doi: 10.1189/jlb.0407259. Epub 2007 Aug 
      20.