PMID- 17709559 OWN - NLM STAT- MEDLINE DCOM- 20071127 LR - 20070821 IS - 1470-1626 (Print) IS - 1470-1626 (Linking) VI - 134 IP - 3 DP - 2007 Sep TI - Beneficial effects of brain-derived neurotropic factor on in vitro maturation of porcine oocytes. PG - 405-14 AB - In an effort to improve the quality of in vitro produced porcine embryos, we investigated the effect of brain-derived neurotropic factor (BDNF), a neurotropin family member, on in vitro maturation (IVM) of porcine oocytes. The expression of BDNF and truncated isoforms of its receptor, tyrosine kinase B (TrkB), and p75 common neurotropin receptor was detected in both follicular cells and metaphase-I stage oocytes by RT-PCR. However, mRNA of full-length TrkB was not found in oocytes although it was detected in follicular cells. The expression pattern of BDNF and TrkB was confirmed by immunohistochemistry. Supplementation with BDNF (30 ng/ml) during IVM significantly (P < 0.05) increased the first polar body extrusion and glutathione levels in oocytes, whereas the effect of BDNF on nuclear maturation was diminished when gonadotropin and epidermal growth factor (EGF) were added to the culture media. However, treatment with BDNF (30 ng/ml) along with EGF (10 ng/ml) in the presence of gonadotropin significantly (P < 0.05) increased the developmental competence of oocytes to the blastocyst stage after both in vitro fertilization (IVF; 29.1% when compared with control, 15.6%) and somatic cell nuclear transfer (SCNT; 13.6% when compared with control, 3%). This appeared to reflect a stimulatory interaction between BDNF and EGF to enhance the cytoplasmic maturation of oocytes to support successful preimplantation development. In conclusion, BDNFenhanced nuclearand cytoplasmic maturation of oocytes by autocrine and/or paracrine signals. Also, when used together with EGF, BDNF increased the developmental potency of embryos after IVF and SCNT, demonstrating an improved in vitro production protocol for porcine oocytes. FAU - Lee, Eugine AU - Lee E AD - SooAm Biotech Research Foundation, Sooambuilding 1027-4, Bangbae3-dong, Seocho-gu, Seoul 137-851, South Korea. FAU - Jeong, Yeon Ik AU - Jeong YI FAU - Park, Seon Mi AU - Park SM FAU - Lee, Jong Yun AU - Lee JY FAU - Kim, Ji Hye AU - Kim JH FAU - Park, Sun Woo AU - Park SW FAU - Hossein, M S AU - Hossein MS FAU - Jeong, Yeon Woo AU - Jeong YW FAU - Kim, Sue AU - Kim S FAU - Hyun, Sang Hwan AU - Hyun SH FAU - Hwang, Woo Suk AU - Hwang WS LA - eng PT - Journal Article PL - England TA - Reproduction JT - Reproduction (Cambridge, England) JID - 100966036 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Gonadotropins, Pituitary) RN - 0 (RNA, Messenger) RN - 62229-50-9 (Epidermal Growth Factor) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/analysis/metabolism/*pharmacology MH - Cells, Cultured MH - Embryo Culture Techniques MH - Embryonic Development MH - Epidermal Growth Factor/pharmacology MH - Female MH - Fertilization in Vitro/veterinary MH - Gonadotropins, Pituitary/pharmacology MH - Immunohistochemistry MH - Metaphase MH - Oocytes/cytology/*metabolism MH - Oogenesis/*drug effects MH - RNA, Messenger/analysis MH - Reverse Transcriptase Polymerase Chain Reaction MH - Signal Transduction/*physiology MH - *Swine EDAT- 2007/08/22 09:00 MHDA- 2007/12/06 09:00 CRDT- 2007/08/22 09:00 PHST- 2007/08/22 09:00 [pubmed] PHST- 2007/12/06 09:00 [medline] PHST- 2007/08/22 09:00 [entrez] AID - 134/3/405 [pii] AID - 10.1530/REP-06-0288 [doi] PST - ppublish SO - Reproduction. 2007 Sep;134(3):405-14. doi: 10.1530/REP-06-0288.