PMID- 1770974
OWN - NLM
STAT- MEDLINE
DCOM- 19920221
LR  - 20190907
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 12
IP  - 5
DP  - 1991 Sep-Oct
TI  - Ferritin: the role of aluminum in ferritin function.
PG  - 413-8
AB  - We previously showed that human brain ferritin (HBF) binds aluminum (Al) in vivo 
      and in vitro and HBF isolated from Alzheimer's brain had more Al bound compared 
      to aged matched controls (7). To further understand the role ferritin may play in 
      Al neurotoxicity, we have studied in vitro the effect of Al on the function of 
      human ferritin isolated from Alzheimer's (AD) and normal brain tissue, and 
      compared the results with other mammalian ferritins. Al causes a 
      concentration-dependent decrease in the initial rate of iron loading into 
      apo-horse spleen and human brain ferritin and the rates were similar for ferritin 
      isolated from both AD and normal brains. The rates of iron release of mammalian 
      ferritins from different tissues were determined: horse spleen much greater than 
      human liver greater than rat brain greater than human brain = rat liver ferritin. 
      The rates of iron release of AD and normal human brain ferritin were similar and 
      were unaffected by preloading with Al. Several mammalian ferritins were compared 
      for their total iron uptake: horse spleen = human liver greater than human brain 
      (normal) = human brain (AD) ferritin. In 20 mM HEPES (pH 6.0) buffer holoferritin 
      is more resistant to precipitation by Al than apoferritin suggesting that 
      holoferritin is a better chelator for nonferrous metal ions.
FAU - Fleming, J T
AU  - Fleming JT
AD  - Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
FAU - Joshi, J G
AU  - Joshi JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 9007-73-2 (Ferritins)
RN  - CPD4NFA903 (Aluminum)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Aluminum/*pharmacology
MH  - Animals
MH  - Brain Chemistry/drug effects
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Ferritins/chemistry/isolation & purification/*metabolism
MH  - Horses
MH  - Humans
MH  - Iron/metabolism/pharmacology
MH  - Liver/metabolism
MH  - Protein Binding/drug effects
MH  - Rats
MH  - Spleen/metabolism
EDAT- 1991/09/01 00:00
MHDA- 1991/09/01 00:01
CRDT- 1991/09/01 00:00
PHST- 1991/09/01 00:00 [pubmed]
PHST- 1991/09/01 00:01 [medline]
PHST- 1991/09/01 00:00 [entrez]
AID - 0197-4580(91)90066-S [pii]
AID - 10.1016/0197-4580(91)90066-s [doi]
PST - ppublish
SO  - Neurobiol Aging. 1991 Sep-Oct;12(5):413-8. doi: 10.1016/0197-4580(91)90066-s.