PMID- 17711410
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20071008
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 70
IP  - 5
DP  - 2007 Nov
TI  - Cytokine gene polymorphisms in Colombian patients with systemic lupus 
      erythematosus.
PG  - 376-82
AB  - Systemic lupus erythematosus (SLE) patients exhibit alterations in cytokine 
      production that may be relevant to SLE pathogenesis. There is evidence that 
      cytokine gene polymorphisms control cytokine production; thus, these 
      polymorphisms may be associated with SLE or its clinical manifestations. To 
      establish the association of tumor necrosis factor alpha (TNF-alpha), 
      transforming growth factor (TGF) beta1, interleukin (IL)-10, and IL-6 gene 
      polymorphisms in Colombian SLE patients and their clinical manifestations, 120 
      SLE patients and 102 healthy controls were studied. Single nucleotide 
      polymorphisms were studied by sequence-specific primers polymerase chain reaction 
      (SSP-PCR) at: TNFalpha-308 (G/A), TGFbeta1 codon 10 (C/T) and codon 25 (G/C), 
      IL-10 -1082 (G/A), -819 (C/T) and -592 (C/A), and IL-6 + 174 (G/C). Human 
      leukocyte antigen (HLA)-DRbeta1 was typed by SSP-PCR. SLE patients had increased 
      frequency of allele C at TGFbeta1 codon 25 (P = 0.0001, odds ratio (OR): 4.25, 
      95% confidence interval (CI): 2.17-8.35) and allele A at TNFalpha-308 (P = 0.0004 
      OR: 3.9, 95% CI: 1.65-5.80) compared with healthy controls. There was higher 
      frequency of GC genotype at TGFbeta1 codon 25 in SLE patients (P < 0.0001). 
      Extended genotypic analysis showed that SLE patients have decreased frequency of 
      TNFalphaLow/TGFbeta1High (0.50) compared with healthy controls (0.80) (P < 
      0.0001). No association was found between these polymorphisms and SLE clinical 
      manifestations except for Sm and Ro autoantibodies that were associated with 
      TNFalpha allele A. There is an association between TNFalpha-308A/TGFbeta1 codon 
      25C with SLE susceptibility in Colombian population. This association may result 
      in a highly inflammatory response with a decrease regulatory function mediated by 
      TNFalpha and TGFbeta1, respectively. The TNFalpha-308A/TGFbeta1 25C genotype may 
      be one component of genetic susceptibility to SLE in Colombian population.
FAU - Guarnizo-Zuccardi, P
AU  - Guarnizo-Zuccardi P
AD  - Grupo de Immunologia Celular e Inmunogenetica, Universidad de Antioquia, 
      Medellin, Colombia.
FAU - Lopez, Y
AU  - Lopez Y
FAU - Giraldo, M
AU  - Giraldo M
FAU - Garcia, N
AU  - Garcia N
FAU - Rodriguez, L
AU  - Rodriguez L
FAU - Ramirez, L
AU  - Ramirez L
FAU - Uribe, O
AU  - Uribe O
FAU - Garcia, L
AU  - Garcia L
FAU - Vasquez, G
AU  - Vasquez G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070817
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Codon)
RN  - 0 (Cytokines)
SB  - IM
MH  - *Alleles
MH  - Codon/genetics
MH  - Colombia
MH  - Cytokines/*genetics
MH  - Female
MH  - *Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Lupus Erythematosus, Systemic/*genetics/pathology
MH  - Male
MH  - *Polymorphism, Single Nucleotide
EDAT- 2007/08/23 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/08/23 09:00
PHST- 2007/08/23 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/08/23 09:00 [entrez]
AID - TAN917 [pii]
AID - 10.1111/j.1399-0039.2007.00917.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Nov;70(5):376-82. doi: 10.1111/j.1399-0039.2007.00917.x. 
      Epub 2007 Aug 17.