PMID- 17711418
OWN - NLM
STAT- MEDLINE
DCOM- 20071120
LR  - 20220309
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 103
IP  - 2
DP  - 2007 Oct
TI  - Robust stimulation of TrkB induces delayed increases in BDNF and Arc mRNA 
      expressions in cultured rat cortical neurons via distinct mechanisms.
PG  - 626-36
AB  - In cultures of rat cortical neurons, we found that stimulation of tyrosine 
      receptor kinase B (TrkB) with brain-derived neurotrophic factor (BDNF) induced a 
      biphasic expression of BDNF exon IV-IX mRNA, which became obvious 1-3 h (primary 
      induction) and 24-72 h (delayed induction) after the stimulation, and 
      characterized the delayed induction in relation to the mRNA expression of 
      activity-regulated cytoskeleton-associated protein (Arc). Withdrawal of BDNF from 
      the medium after stimulation for 3 h allowed the delayed induction, which was 
      caused at the transcriptional level and dependent upon the initial contact 
      between exogenously added BDNF and TrkB, the effect of which was time- and 
      dose-dependent. The primary induction was controlled by the extracellular 
      signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) whereas the 
      secondary induction by the calcium (Ca(2+)) signaling pathway. The enhanced Arc 
      or Zif268 mRNA expression was controlled by activation of the ERK/MAPK pathway, 
      both of which were repressed by blocking the binding of endogenously synthesized 
      BDNF to TrkB. Thus, robust stimulation of TrkB autonomously induces delayed BDNF 
      mRNA expression in an activity-dependent manner in rat cortical neurons, 
      resulting in the stimulation of Arc mRNA expression through endogenously 
      synthesized BDNF, the process being orchestrated by the Ca(2+) and ERK/MAPK 
      signaling pathways.
FAU - Yasuda, Makoto
AU  - Yasuda M
AD  - Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Graduate 
      School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, 
      Toyama, Japan.
FAU - Fukuchi, Mamoru
AU  - Fukuchi M
FAU - Tabuchi, Akiko
AU  - Tabuchi A
FAU - Kawahara, Masahiro
AU  - Kawahara M
FAU - Tsuneki, Hiroshi
AU  - Tsuneki H
FAU - Azuma, Yuko
AU  - Azuma Y
FAU - Chiba, Yusuke
AU  - Chiba Y
FAU - Tsuda, Masaaki
AU  - Tsuda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070817
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (activity regulated cytoskeletal-associated protein)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Cytoskeletal Proteins/*biosynthesis
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Extracellular Signal-Regulated MAP Kinases/biosynthesis/genetics/metabolism
MH  - Genes, Immediate-Early/drug effects
MH  - Immunoblotting
MH  - Indicators and Reagents
MH  - Luciferases/genetics
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Neurons/drug effects/*metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*agonists
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
MH  - Transcriptional Activation/drug effects
MH  - Transfection
EDAT- 2007/08/23 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/23 09:00
PHST- 2007/08/23 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/23 09:00 [entrez]
AID - JNC4851 [pii]
AID - 10.1111/j.1471-4159.2007.04851.x [doi]
PST - ppublish
SO  - J Neurochem. 2007 Oct;103(2):626-36. doi: 10.1111/j.1471-4159.2007.04851.x. Epub 
      2007 Aug 17.