PMID- 17711772
OWN - NLM
STAT- MEDLINE
DCOM- 20071001
LR  - 20161124
IS  - 0707-7270 (Electronic)
IS  - 0381-6605 (Linking)
VI  - 36
IP  - 3
DP  - 2007 Jun
TI  - Significance and mechanism of microsatellite instability in laryngeal squamous 
      cell carcinoma.
PG  - 168-74
AB  - OBJECTIVE: To evaluate the significance and mechanism of microsatellite 
      instability (MSI) in laryngeal squamous cell carcinoma (LSCC). METHODS: We 
      investigated the expression frequency and clinical significance of MSI in 50 LSCC 
      patients. The status of MSI was evaluated by using microdissection, polymerase 
      chain reaction, single-strand length polymorphism, and silver staining. Five 
      markers on chromosomes 1p, 3p, 5q, 9p, and 17p were used. Two of the six 
      components of mismatch repair (MMR)-hMLH1 and hMSH2-were investigated by an 
      immunohistochemical approach. RESULTS: The informative case numbers of the five 
      markers (D17S796, D3S3544, D5S656, D1S375, D9S162) were 44, 42, 45, 44, and 40 in 
      all 50 cases, respectively. The incidence of MSI on D17S796 (TP53) was 20.5% (9 
      of 44), on D3S3544 (FHIT) was 14.3% (6 of 42), on D5S656 (APC) was 31.1% (14 of 
      45), on D1S375 (BCAR3) was 20.5% (9 of 44), and on D9S162 (CDKN2A) was 15.0% (6 
      of 40). Although there was no relationship between MSI status and age, gender, 
      smoking history, tumour location, tumour differentiation, and T stage (p > .05), 
      there was a strong relationship between MSI and relapse condition (p < .01). 
      Also, MSI status correlated with MMR expression to some degree (p < .01). But it 
      was common that negative and positive staining of MMR coexisted on the same 
      slide. CONCLUSION: MSI and abnormal MMR may contribute to the carcinogenesis of a 
      subset of LSCC. MSI may be a characteristic signal of tumour recurrence.
FAU - Zhigang, Huang
AU  - Zhigang H
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Beijing Institute of 
      Otorhinolaryngology, Beijing Tongren Hospital, Capital University of Medical 
      Science, Beijing, China. haohan1992@163.com <haohan1992@163.com>
FAU - Demin, Han
AU  - Demin H
FAU - Han, Gao
AU  - Han G
FAU - Erzhong, Fan
AU  - Erzhong F
FAU - Xiaohong, Chen
AU  - Xiaohong C
FAU - Hongbo, Xu
AU  - Hongbo X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - J Otolaryngol
JT  - The Journal of otolaryngology
JID - 7610513
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MLH1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - EC 3.6.1.3 (MSH2 protein, human)
RN  - EC 3.6.1.3 (MutL Protein Homolog 1)
RN  - EC 3.6.1.3 (MutS Homolog 2 Protein)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Differentiation
MH  - DNA Mismatch Repair
MH  - Epithelium/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Microdissection
MH  - *Microsatellite Instability
MH  - Middle Aged
MH  - MutL Protein Homolog 1
MH  - MutS Homolog 2 Protein/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
EDAT- 2007/08/23 09:00
MHDA- 2007/10/02 09:00
CRDT- 2007/08/23 09:00
PHST- 2007/08/23 09:00 [pubmed]
PHST- 2007/10/02 09:00 [medline]
PHST- 2007/08/23 09:00 [entrez]
PST - ppublish
SO  - J Otolaryngol. 2007 Jun;36(3):168-74.