PMID- 17712116
OWN - NLM
STAT- MEDLINE
DCOM- 20071102
LR  - 20091119
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 15
IP  - 8
DP  - 2007 Aug
TI  - Macrophage-colony stimulating factor in obese adipose tissue: studies with 
      heterozygous op/+ mice.
PG  - 1988-95
AB  - OBJECTIVE: We examined the gene expression of macrophage-colony stimulating 
      factor (M-CSF) in mice with diet-induced obesity and in genetically obese mice. 
      We also examined the effect of decreased M-CSF signaling on the susceptibility to 
      obesity and macrophage recruitment into the adipose tissue of mice. RESEARCH 
      METHODS AND PROCEDURES: The adipose tissue from mice with diet-induced obesity, 
      obese KKA(y) mice, and ob/ob obese mice was used for RNA preparation. Production 
      of M-CSF and monocyte chemoattractant protein-1 (MCP-1) was examined by 
      quantitative real-time polymerase chain reaction (PCR) and enzyme-linked 
      immunosorbent assay. The op/+ heterozygous mice, with decreased functional M-CSF 
      expression, were placed on a high-fat diet or crossed with KKA(y) mice to study 
      the susceptibility to obesity. The gene expression of macrophage markers in 
      adipose tissue was examined. RESULTS: The expression of M-CSF was not 
      significantly changed in mice on a high-fat diet or in either type of genetic 
      obesity (KKA(y) or ob/ob mice). No change in the degree of obesity or 
      macrophage-related gene expression (F4/80, CD68, and MCP-1) in the adipose tissue 
      was observed in op/+ mice compared with +/+ control mice, which were either 
      treated with a high-fat diet or crossed with KKA(y) mice. DISCUSSION: This study 
      demonstrated that there was no significant change in the expression of M-CSF in 
      the adipose tissue from obese mice and only a minor phenotypic change, such as 
      macrophage infiltration, in the adipose tissue from op/+ mice, suggesting that 
      M-CSF does not play a major role in macrophage recruitment in the adipose tissue 
      of obese mice.
FAU - Sugita, Satoshi
AU  - Sugita S
AD  - Department of Molecular Medicine and Metabolism, Medical Research Institute, 
      Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Tokyo 101-0062, 
      Japan.
FAU - Kamei, Yasutomi
AU  - Kamei Y
FAU - Oka, Jun-Ichiro
AU  - Oka J
FAU - Suganami, Takayoshi
AU  - Suganami T
FAU - Ogawa, Yoshihiro
AU  - Ogawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (monocyte-macrophage differentiation antigen)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/biosynthesis/genetics
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Crosses, Genetic
MH  - Histocytochemistry
MH  - Macrophage Colony-Stimulating Factor/*biosynthesis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Obesity/genetics/*immunology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptor, Macrophage Colony-Stimulating Factor/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Subcutaneous Fat/*immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 2007/08/23 09:00
MHDA- 2007/11/06 09:00
CRDT- 2007/08/23 09:00
PHST- 2007/08/23 09:00 [pubmed]
PHST- 2007/11/06 09:00 [medline]
PHST- 2007/08/23 09:00 [entrez]
AID - 15/8/1988 [pii]
AID - 10.1038/oby.2007.237 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2007 Aug;15(8):1988-95. doi: 10.1038/oby.2007.237.