PMID- 17713013
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20190328
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 601
DP  - 2007
TI  - Differences in dendritic cell activation and distribution after intravenous, 
      intraperitoneal, and subcutaneous injection of lymphoma cells in mice.
PG  - 257-64
AB  - Dendritic cells (DCs) are key antigen-presenting cells (APCs) for initiating 
      immune responses. However, in recent years, several groups have shown the 
      defective function of DCs in tumor-bearing mice and in cancer patients. Our aim 
      was to study the effects of lymphoma on DC differentiation and maturation and to 
      assess the input of the tumor microenvironment and intravasation of tumor cells 
      on DC precursors. EL-4 lymphoma cells were administrated via different routes 
      (intraperitoneal, subcutaneous, and intravenous) and DC phenotype was 
      investigated. Bone marrow-derived DCs and APCs obtained from the spleen were 
      examined by flow cytometry, and immunohistochemical analysis of lymphoma, lungs, 
      livers, and spleens was also performed. Intravenous administration of lymphoma 
      cells induced suppression of DC differentiation and maturation assessed as a 
      significant decrease of the IAb, CD80, CD86, CD11b, and CD11c expression on DCs 
      and IAb on splenic APCs. Upregulation of APC differentiation was observed in 
      animals after subcutaneous and intraperitoneal administration of lymphoma cells 
      determined as increased expression of CD40 and CD86 in spleen APCs. These data 
      suggest that the development of antitumor immune response might differ in the 
      host receiving tumor vaccines via different injection routes.
FAU - Sevko, Alexandra L
AU  - Sevko AL
AD  - R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, 
      Ukrainian Academy of Sciences, Kyiv, Ukraine.
FAU - Barysik, Nadzeya
AU  - Barysik N
FAU - Perez, Lori
AU  - Perez L
FAU - Shurin, Michael R
AU  - Shurin MR
FAU - Gerein, Valentin
AU  - Gerein V
LA  - eng
GR  - 5R01 CA84270/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Antineoplastic Agents)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/metabolism
MH  - Antineoplastic Agents/metabolism
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Dendritic Cells/cytology/*metabolism
MH  - Flow Cytometry
MH  - Infusions, Intravenous
MH  - Infusions, Parenteral
MH  - Injections, Subcutaneous
MH  - Lymphoma/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - Spleen/cytology
EDAT- 2007/08/24 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
AID - 10.1007/978-0-387-72005-0_27 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2007;601:257-64. doi: 10.1007/978-0-387-72005-0_27.