PMID- 17713439
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20070823
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 84
IP  - 4
DP  - 2007 Aug 27
TI  - Human leukocyte antigen class I epitopes: update to 103 total epitopes, including 
      the C locus.
PG  - 532-40
AB  - BACKGROUND: Epitopes of human leukocyte antigen (HLA) are the sites to which the 
      antibodies bind. We identify here 103 HLA class I epitopes shared by groups of 
      class I antigens. In particular, our emphasis was on identifying epitopes 
      exclusive to the C-locus antigens or interlocus epitopes among A, B, and C 
      antigens. The use of monoclonal antibodies or alloantibodies eluted from HLA 
      recombinant single antigen cell lines tested with a panel of single antigen beads 
      have proved very useful in the identification of the epitopes. METHODS: 
      Alloantibodies absorbed onto then eluted from HLA single antigen cell lines and 
      monoclonal antibodies were tested with a panel of 95 A-, B-, and C- single 
      antigen beads and the HLA specificities determined. Each epitope was defined by 
      amino acids shared exclusively by the positive antigens for each antibody. 
      RESULTS: In addition to the 58 A and B class I epitopes identified in an earlier 
      study, we add 45 more new A, B, C epitopes including, for the first time, 
      epitopes found on C locus antigens. CONCLUSION: Beads bearing single antigens 
      tested with monoclonal or eluted alloantibodies proved very powerful in 
      identifying epitopes shared among HLA antigens. These epitopes are the targets of 
      the antibodies. Antibody specificities to non-donor-specific antigens, often 
      found in sera of transplant patients, can now be understood as reactions to 
      epitopes shared with the donor specific antigens. The importance of identifying 
      these epitopes is that they may be the "transplantation antigens" responsible for 
      antibody-mediated transplant rejection.
FAU - El-Awar, Nadim R
AU  - El-Awar NR
AD  - One Lambda Inc., Research II, 11570 W. Olympic Boulevard, Los Angeles, CA 90064, 
      USA. nelawar@onelambda.com
FAU - Akaza, Tatsuya
AU  - Akaza T
FAU - Terasaki, Paul I
AU  - Terasaki PI
FAU - Nguyen, Anh
AU  - Nguyen A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antibody Specificity/immunology
MH  - Epitopes/*genetics/immunology
MH  - Genes, MHC Class I/*genetics/immunology
MH  - Graft Rejection/genetics/immunology
MH  - HLA-A Antigens/genetics/immunology
MH  - HLA-B Antigens/genetics/immunology
MH  - HLA-C Antigens/*genetics/immunology
MH  - Humans
MH  - Isoantibodies/immunology
MH  - Transplantation Immunology/genetics/immunology
EDAT- 2007/08/24 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
AID - 00007890-200708270-00015 [pii]
AID - 10.1097/01.tp.0000278721.97037.1e [doi]
PST - ppublish
SO  - Transplantation. 2007 Aug 27;84(4):532-40. doi: 
      10.1097/01.tp.0000278721.97037.1e.