PMID- 17714401
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20081121
IS  - 1364-5072 (Print)
IS  - 1364-5072 (Linking)
VI  - 103
IP  - 3
DP  - 2007 Sep
TI  - Phylogenetic analysis of intestinal bacteria in the Chinese mitten crab 
      (Eriocheir sinensis).
PG  - 675-82
AB  - AIMS: To identify the dominant intestinal bacteria in the Chinese mitten crab, 
      and to investigate the differences in the intestinal bacteria between pond-raised 
      and wild crabs. METHODS AND RESULTS: The diversity of intestinal bacteria in the 
      Chinese mitten crabs was investigated by denaturing gradient gel electrophoresis 
      (DGGE) fingerprinting, 16S rRNA gene clone library analysis and real-time 
      quantitative PCR. The principal component analysis of DGGE profiles indicated 
      that substantial intersubject variations existed in intestinal bacteria in 
      pond-raised crab. The sequencing of 16S rRNA genes revealed that 90-95% of the 
      phylotypes in the clone libraries were affiliated with Proteobacteria and 
      Bacteroidetes. Some genera were identified as unique in wild crabs and in 
      pond-raised crabs, whereas Bacteroidetes was found to be common in all sampled 
      crab groups. Real-time quantitative PCR indicated that the abundance of 
      Bacteroides and the total bacterial load were approximately four-to-10 times 
      higher in pond-raised crabs than in wild crabs. A significant portion of the 
      phylotypes shared low similarity with previously sequenced organisms, indicating 
      that the bacteria in the gut of Chinese mitten crabs are yet to be described. 
      CONCLUSIONS: The intestinal bacteria of pond-raised crabs showed higher 
      intersubject variation, total diversity and abundance than that observed in wild 
      crabs. The high proportion of the clones of Proteobacteria and Bacteroidetes in 
      the clone library is an indication that these bacteria may be the dominant 
      population in the gut of the Chinese mitten crab. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: This study demonstrated obvious differences in the intestinal bacterial 
      composition of pond-raised crabs and wild crabs. This knowledge will increase our 
      understanding of the effects of aquaculture operations on bacterial community 
      composition in the crab gut and provide necessary data for the development of 
      probiotic products for crab cultivation.
FAU - Li, K
AU  - Li K
AD  - Laboratory of Molecular Microbial Ecology and Ecogenomics, College of Life 
      Science and Biotechnology, Shanghai Jiaotong University, Shanghai, China.
FAU - Guan, W
AU  - Guan W
FAU - Wei, G
AU  - Wei G
FAU - Liu, B
AU  - Liu B
FAU - Xu, J
AU  - Xu J
FAU - Zhao, L
AU  - Zhao L
FAU - Zhang, Y
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/*genetics/isolation & purification
MH  - Bacteroides/genetics/isolation & purification
MH  - Brachyura/*microbiology
MH  - China
MH  - DNA, Bacterial/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - *Food Microbiology
MH  - Genetic Variation/genetics
MH  - Intestines/*microbiology
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Proteobacteria/genetics/isolation & purification
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Water Microbiology
EDAT- 2007/08/24 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
AID - JAM3295 [pii]
AID - 10.1111/j.1365-2672.2007.03295.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2007 Sep;103(3):675-82. doi: 10.1111/j.1365-2672.2007.03295.x.