PMID- 17714439
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20220311
IS  - 1467-7652 (Electronic)
IS  - 1467-7644 (Linking)
VI  - 5
IP  - 6
DP  - 2007 Nov
TI  - Development of transgenic rice seed accumulating a major Japanese cedar pollen 
      allergen (Cry j 1) structurally disrupted for oral immunotherapy.
PG  - 815-26
AB  - Rice seed-based edible vaccines expressing T-cell epitope peptides derived from 
      Japanese cedar major pollen allergens have been used to successfully suppress 
      allergen-specific Th2-mediated immunoglobulin E (IgE) responses in mouse 
      experiments. In order to further expand the application of seed-based 
      allergen-specific immunotherapy for controlling Japanese cedar pollinosis, we 
      generated transgenic rice plants that specifically express recombinant Cry j 1 
      allergens in seeds. Cry j 1 allergens give low specific IgE-binding activity but 
      contain all of the T-cell epitopes. The allergens were expressed directly or as a 
      protein fusion with the major rice storage protein glutelin. Fusion proteins 
      expressed under the control of the strong rice endosperm-specific GluB-1 promoter 
      accumulated in rice endosperm tissue up to 15% of total seed protein. The fusion 
      proteins aggregated with cysteine-rich prolamin and were deposited in endoplasmic 
      reticulum-derived protein body I. The production of transgenic rice expressing 
      structurally disrupted Cry j 1 peptides with low IgE binding activity but 
      spanning the entire Cry j1 region can be used as a universal, safe and effective 
      tolerogen for rice seed-based oral immunotherapy for cedar pollen allergy in 
      humans and other mammals.
FAU - Yang, Lijun
AU  - Yang L
AD  - Transgenic Crop Research and Development Center, National Institute of 
      Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.
FAU - Suzuki, Kazuya
AU  - Suzuki K
FAU - Hirose, Sakiko
AU  - Hirose S
FAU - Wakasa, Yuhya
AU  - Wakasa Y
FAU - Takaiwa, Fumio
AU  - Takaiwa F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070822
PL  - England
TA  - Plant Biotechnol J
JT  - Plant biotechnology journal
JID - 101201889
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Cry j I protein, Cryptomeria japonica)
RN  - 0 (Plant Proteins)
RN  - 0 (Prolamins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 8002-80-0 (Glutens)
SB  - IM
MH  - Administration, Oral
MH  - Allergens/genetics/immunology/*metabolism
MH  - Antigens, Plant
MH  - Blotting, Southern
MH  - Cryptomeria/genetics
MH  - Genetic Vectors
MH  - Glutens/metabolism
MH  - Oryza/genetics/*metabolism
MH  - Plant Proteins/genetics/immunology/*metabolism
MH  - Plants, Genetically Modified/*metabolism
MH  - Prolamins
MH  - Recombinant Fusion Proteins/immunology/*metabolism
MH  - Seeds/*metabolism
MH  - Vaccination
EDAT- 2007/08/24 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
AID - PBI287 [pii]
AID - 10.1111/j.1467-7652.2007.00287.x [doi]
PST - ppublish
SO  - Plant Biotechnol J. 2007 Nov;5(6):815-26. doi: 10.1111/j.1467-7652.2007.00287.x. 
      Epub 2007 Aug 22.