PMID- 17715060
OWN - NLM
STAT- MEDLINE
DCOM- 20071017
LR  - 20240316
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 104
IP  - 35
DP  - 2007 Aug 28
TI  - Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal 
      exocytosis in astrocytes.
PG  - 14151-6
AB  - Optical imaging of individual vesicle exocytosis is providing new insights into 
      the mechanism and regulation of secretion by cells. To study calcium-triggered 
      secretion from astrocytes, we used acridine orange (AO) to label vesicles. 
      Although AO is often used for imaging exocytosis, we found that imaging vesicles 
      labeled with AO can result in their photolysis. Here, we define experimental and 
      analytical approaches that permit us to distinguish unambiguously between fusion, 
      leakage, and lysis of individual vesicles. We have used this approach to 
      demonstrate that lysosomes undergo calcium-triggered exocytosis in astrocytes.
FAU - Jaiswal, Jyoti K
AU  - Jaiswal JK
AD  - The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA. 
      jaiswaj@rockefeller.edu
FAU - Fix, Marina
AU  - Fix M
FAU - Takano, Takahiro
AU  - Takano T
FAU - Nedergaard, Maiken
AU  - Nedergaard M
FAU - Simon, Sanford M
AU  - Simon SM
LA  - eng
GR  - P20 GM072015/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070821
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/*physiology
MH  - Calcium/*physiology
MH  - Exocytosis
MH  - Lysosomes/*physiology/ultrastructure
MH  - Rats
PMC - PMC1955787
COIS- The authors declare no conflict of interest.
EDAT- 2007/08/24 09:00
MHDA- 2007/10/18 09:00
PMCR- 2008/02/28
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/10/18 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
PHST- 2008/02/28 00:00 [pmc-release]
AID - 0704935104 [pii]
AID - 7269 [pii]
AID - 10.1073/pnas.0704935104 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14151-6. doi: 
      10.1073/pnas.0704935104. Epub 2007 Aug 21.