PMID- 17715229
OWN - NLM
STAT- MEDLINE
DCOM- 20071127
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 21
DP  - 2007 Nov
TI  - Differential effects of mutations in NS4B on West Nile virus replication and 
      inhibition of interferon signaling.
PG  - 11809-16
AB  - West Nile virus (WNV) is a human pathogen that can cause symptomatic infections 
      associated with meningitis and encephalitis. Previously, we demonstrated that 
      replication of WNV inhibits the interferon (IFN) signal transduction pathway by 
      preventing the accumulation of phosphorylated Janus kinase 1 (JAK1) and tyrosine 
      kinase 2 (Tyk2) (J. T. Guo et al., J. Virol. 79:1343-1350, 2005). Through a 
      genetic analysis, we have now identified a determinant on the nonstructural 
      protein 4B (NS4B) that controls IFN resistance in HeLa cells expressing 
      subgenomic WNV replicons lacking the structural genes. However, in the context of 
      infectious genomes, the same determinant did not influence IFN signaling. Thus, 
      our results indicate that NS4B may be sufficient to inhibit the IFN response in 
      replicon cells and suggest a role for structural genes, or as yet unknown 
      interactions, in the inhibition of the IFN signaling pathway during WNV 
      infections.
FAU - Evans, Jared D
AU  - Evans JD
AD  - Fox Chase Cancer Center, Institute for Cancer Research, 333 Cottman Avenue, 
      Philadelphia, PA 19111, USA. jared.evans@fccc.edu
FAU - Seeger, Christoph
AU  - Seeger C
LA  - eng
SI  - GENBANK/EF536932
GR  - T32 CA009035/CA/NCI NIH HHS/United States
GR  - T32 CA 09035-30/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070822
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 9008-11-1 (Interferons)
RN  - EC 2.7.10.2 (JAK1 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 1)
RN  - EC 2.7.10.2 (TYK2 Kinase)
RN  - EC 2.7.10.2 (TYK2 protein, human)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - *Gene Expression Regulation
MH  - HeLa Cells/*virology
MH  - Humans
MH  - Interferons/*metabolism
MH  - Janus Kinase 1/*metabolism
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Phosphorylation
MH  - Signal Transduction
MH  - TYK2 Kinase/*metabolism
MH  - Viral Nonstructural Proteins/chemistry/*genetics
MH  - beta-Lactamases/metabolism
PMC - PMC2168815
EDAT- 2007/08/24 09:00
MHDA- 2007/12/06 09:00
PMCR- 2008/03/01
CRDT- 2007/08/24 09:00
PHST- 2007/08/24 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/24 09:00 [entrez]
PHST- 2008/03/01 00:00 [pmc-release]
AID - JVI.00791-07 [pii]
AID - 0791-07 [pii]
AID - 10.1128/JVI.00791-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Nov;81(21):11809-16. doi: 10.1128/JVI.00791-07. Epub 2007 Aug 22.