PMID- 17717713
OWN - NLM
STAT- MEDLINE
DCOM- 20080221
LR  - 20181113
IS  - 0024-4201 (Print)
IS  - 0024-4201 (Linking)
VI  - 42
IP  - 11
DP  - 2007 Nov
TI  - Characteristic oxidation products of choline plasmalogens are detectable in 
      cattle and roe deer spermatozoa by MALDI-TOF mass spectrometry.
PG  - 991-8
AB  - Plasmalogens (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphocholines and 
      -phosphoethanolamines) are important constituents of spermatozoa membranes and 
      possess significant antioxidative properties. This particularly holds as 
      plasmalogens from spermatozoa also possess a very high content of highly 
      unsaturated fatty acyl residues (especially 22:6). The organic spermatozoa 
      extracts of two different ruminants (cattle and roe deer) were analyzed for their 
      contents of characteristic choline plasmalogen oxidation products by 
      matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass 
      spectrometry. It will be shown that 
      1-hydroxy-2-docosahexaenoyl-sn-glycero-3-phosphocholine (LPC 22:6) and formyl-LPC 
      22:6 are reliable measures of lipid oxidation of spermatozoa and allow, 
      accordingly, conclusions about the storage conditions. All data on spermatozoa 
      were also confirmed by the investigation of the oxidation behavior of selected 
      reference compounds. It will be shown that, equally if plasmalogens or diacyl PC 
      species are used, oxidation takes place primarily at the double bond next to the 
      glycerol backbone. These data were additionally confirmed by recording the 
      corresponding post source decay (PSD) fragment ion spectra.
FAU - Fuchs, Beate
AU  - Fuchs B
AD  - Faculty of Medicine, Institute of Medical Physics and Biophysics, University of 
      Leipzig, Hartelstr. 16-18, 04107, Leipzig, Germany.
FAU - Muller, Karin
AU  - Muller K
FAU - Goritz, Frank
AU  - Goritz F
FAU - Blottner, Steffen
AU  - Blottner S
FAU - Schiller, Jurgen
AU  - Schiller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070824
PL  - United States
TA  - Lipids
JT  - Lipids
JID - 0060450
RN  - 0 (1-hydroxy-2-hexadecanoyl-sn-glycero-3-phosphocholine)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - 0 (Plasmalogens)
RN  - 0 (choline plasmalogens)
SB  - IM
MH  - Animals
MH  - *Cattle
MH  - Cryopreservation/veterinary
MH  - *Deer
MH  - Lipid Peroxidation
MH  - Lysophosphatidylcholines/analysis
MH  - Male
MH  - Oxidation-Reduction
MH  - Phospholipids/chemistry
MH  - Plasmalogens/*analysis/chemistry
MH  - Semen Preservation/veterinary
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spermatozoa/*chemistry
EDAT- 2007/08/25 09:00
MHDA- 2008/02/22 09:00
CRDT- 2007/08/25 09:00
PHST- 2007/06/23 00:00 [received]
PHST- 2007/07/25 00:00 [accepted]
PHST- 2007/08/25 09:00 [pubmed]
PHST- 2008/02/22 09:00 [medline]
PHST- 2007/08/25 09:00 [entrez]
AID - 10.1007/s11745-007-3108-7 [doi]
PST - ppublish
SO  - Lipids. 2007 Nov;42(11):991-8. doi: 10.1007/s11745-007-3108-7. Epub 2007 Aug 24.