PMID- 17718120
OWN - NLM
STAT- MEDLINE
DCOM- 20070912
LR  - 20180217
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 51
IP  - 4
DP  - 2007 Jul-Aug
TI  - Immunocytochemistry and fluorescence in situ hybridization in HER-2/neu status in 
      cell block preparations.
PG  - 552-7
AB  - OBJECTIVE: Recent studies have validated the use of cytologic materials to 
      determine HER-2/neu status. Good concordance has been shown between results 
      obtained by immunocytochemistry (ICC), immunohistochemistry (IHC), fluorescence 
      in situ hybridization (FISH) on cytologic and surgical specimens. However, the 
      utility of cytologic cell block material in determining HER-2/neu status has not 
      been reported and is the subject of this study. STUDY DESIGN: HER-2/neu status 
      was determined in 25 cases of primary or metastatic breast carcinoma by IHC and 
      FISH. All cases were formalin-fixed, paraffin-embedded (FPPE) cell block 
      preparations. ICC was performed using monoclonal antibodies TAB250 (Zymed) and 
      CB11 (Novacastra Laboratories). FISH analysis was performed using the PathVysion 
      HER-2 probe kit (Vysis, Inc.). Results of ICC and FISH were compared in each 
      case. RESULTS: Of 25 cases studied, 17 showed no protein overexpression or 
      amplification. Five cases showed protein overexpression and amplification. The 
      remaining 3 cases showed 2+ staining intensity by ICC in 10, 20, and 50% of 
      carcinoma cells, respectively, and all demonstrated lack of amplification. 
      CONCLUSION: Immunocytochemistry performed on FFPE cell block material is a 
      reliable method for determining HER-2/neu status in cytologic specimens. We 
      recommend routine preparation of FFPE cell block material in instances of 
      suspected primary of metastatic breast carcinoma.
FAU - Shin, Sandra J
AU  - Shin SJ
AD  - Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell 
      University, New York, New York 10021, USA. sjshin@med.cornell.edu
FAU - Chen, Beiyun
AU  - Chen B
FAU - Hyjek, Elizabeth
AU  - Hyjek E
FAU - Vazquez, Madeline
AU  - Vazquez M
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/pathology
MH  - Cytological Techniques/*methods
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Neoplasm Metastasis
MH  - Peritoneal Neoplasms/pathology
MH  - Receptor, ErbB-2/*metabolism
EDAT- 2007/08/28 09:00
MHDA- 2007/09/13 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2007/09/13 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - 10.1159/000325793 [doi]
PST - ppublish
SO  - Acta Cytol. 2007 Jul-Aug;51(4):552-7. doi: 10.1159/000325793.