PMID- 17718844
OWN - NLM
STAT- MEDLINE
DCOM- 20071206
LR  - 20141120
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 45
IP  - 3
DP  - 2007 Sep
TI  - The first molecular analysis of clinical isolates of VanA-type 
      vancomycin-resistant Enterococcus faecium strains in Mainland China.
PG  - 307-12
AB  - AIMS: The aim of this study was to examine two VanA-type vancomycin-resistant 
      Enterococcus faecium (VRE) strains that had been isolated from patients resident 
      in mainland China. This is the first molecular analysis of clinical VRE strains 
      being isolated in mainland China. METHODS AND RESULTS: Two VanA-type VRE isolates 
      were isolated from in-patients at hospitals located in the Chinese cities Beijing 
      and Dalian and were designated C264 and I125. The plasmids pC264V (40 kbp) and 
      pI125V (370 kbp) that were isolated from C264 and I125, respectively, carried a 
      Tn1546-like element encoding VanA resistance. The vancomycin-resistant plasmids 
      pC264V and pI125V were transferred by filter mating at frequencies of 10(-7) and 
      10(-4) respectively. Sequence analysis of pC264V revealed that two IS1216V 
      sequences and an IS1542 sequence were present within the Tn1546-like element. 
      pI125V had two IS1216V insertions in the Tn1546-like element. CONCLUSIONS: The 
      two VanA-type vancomycin-resistant E. faecium (VRE) strains C264 and I125 were 
      isolated from in-patients in Chinese hospitals. The vancomycin-resistant 
      conjugative plasmids pC264V and pI125V plasmids isolated from these strains 
      carried the Tn1546-like element. The Tn1546-like element was found to contain the 
      insertion sequences IS1216V and IS1542. SIGNIFICANCE AND IMPACT OF THE STUDY: 
      This is the first molecular analysis of VanA-type VRE strains from patients 
      resident in mainland China.
FAU - Zheng, B
AU  - Zheng B
AD  - Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, 
      China.
FAU - Tomita, H
AU  - Tomita H
FAU - Xiao, Y H
AU  - Xiao YH
FAU - Ike, Y
AU  - Ike Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (VanA ligase, Bacteria)
RN  - 6Q205EH1VU (Vancomycin)
RN  - EC 6.1.- (Carbon-Oxygen Ligases)
SB  - IM
MH  - Anti-Bacterial Agents/metabolism/pharmacology
MH  - Bacterial Proteins/genetics
MH  - Carbon-Oxygen Ligases/genetics
MH  - China
MH  - DNA Transposable Elements
MH  - DNA, Bacterial/metabolism
MH  - Enterococcus faecium/*drug effects/*genetics/isolation & purification
MH  - Genes, Bacterial/drug effects
MH  - Humans
MH  - Models, Genetic
MH  - Vancomycin/metabolism/pharmacology
MH  - *Vancomycin Resistance
EDAT- 2007/08/28 09:00
MHDA- 2007/12/07 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2007/12/07 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - LAM2191 [pii]
AID - 10.1111/j.1472-765X.2007.02191.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2007 Sep;45(3):307-12. doi: 
      10.1111/j.1472-765X.2007.02191.x.