PMID- 17719190
OWN - NLM
STAT- MEDLINE
DCOM- 20081106
LR  - 20080825
IS  - 0378-4320 (Print)
IS  - 0378-4320 (Linking)
VI  - 108
IP  - 1-2
DP  - 2008 Oct
TI  - Karyological diagnosis of Cebus (Primates, Platyrrhini) in captivity: detection 
      of hybrids and management program applications.
PG  - 66-78
AB  - Genetic data are very important for conservation programs in wild population as 
      well as in captive conditions. Primates in zoos or breeding centers are often 
      maintained in groups without geographic origin or genetic heritage information. 
      These lead to the incorrect assignment of species and introduce an artificial 
      reproductive barrier, which in turn constitutes inadequate management of the 
      colonies. A karyological analysis of specimens from a Primate Reproduction 
      Center, considered as Cebus apella (Platyrrhini), was performed. Cell cultures 
      were conducted from peripheral blood samples following standard cytogenetic 
      methods. A fluorescence in situ hybridization (FISH) procedure was applied in 
      mitotic metaphases using two probes: A specific probe of the extracentromeric 
      heterochromatin (He+) of Cebus, and a human chromosome 21 probe. The latter was 
      chosen due to the known homeology with the euchromatic region limiting with 
      11qHe+ of Cebus. The species status was determined for at least half of the 
      animals and identified a hybrid specimen using this combined FISH protocol. This 
      procedure is an accurate diagnostic methodology for taxonomic determinations and, 
      therefore can be used for management of reproduction in colonies.
FAU - Nieves, M
AU  - Nieves M
AD  - Grupo de Investigacion en Biologia Evolutiva (GIBE), Dpto. Ecologia, Genetica y 
      Evolucion, FCEyN-UBA, Ciudad Universitaria (C1428 EHA), Ciudad Autonoma de Buenos 
      Aires, Argentina. maenieves@yahoo.com
FAU - Mendez, G
AU  - Mendez G
FAU - Ortiz, A
AU  - Ortiz A
FAU - Muhlmann, M
AU  - Muhlmann M
FAU - Mudry, M D
AU  - Mudry MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070721
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
SB  - IM
MH  - Animals
MH  - Animals, Zoo
MH  - Cebus/*classification/*genetics
MH  - Female
MH  - Hybridization, Genetic/*genetics
MH  - Karyotyping/*veterinary
MH  - Male
MH  - Phenotype
EDAT- 2007/08/28 09:00
MHDA- 2008/11/07 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/04/27 00:00 [received]
PHST- 2007/07/07 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2008/11/07 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - S0378-4320(07)00254-0 [pii]
AID - 10.1016/j.anireprosci.2007.07.006 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2008 Oct;108(1-2):66-78. doi: 10.1016/j.anireprosci.2007.07.006. 
      Epub 2007 Jul 21.