PMID- 17720133
OWN - NLM
STAT- MEDLINE
DCOM- 20071113
LR  - 20131121
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 466
IP  - 1
DP  - 2007 Oct 1
TI  - ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in 
      breast cancer (MCF-7) cells.
PG  - 15-23
AB  - In the breast tumor cell line MCF-7, extracellular nucleotides induce transient 
      elevations in intracellular calcium concentration ([Ca(2+)](i)). In this study we 
      show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress 
      leading to an additional transient Ca(2+) influx. ATP> or =ATPgamma-S> or 
      =UTP>>>ADP=ADPbeta-S elevate [Ca(2+)](i), proving the presence of P2Y(2)/P2Y(4) 
      purinergic receptor subtypes. In addition, cell stimulation with ATP, ATPgamma-S 
      or UTP but not ADPbeta-S induced the phosphorylation of ERK1/2, p38 and JNK1/2 
      mitogen activated protein kinases (MAPKs). The use of Gd(3+), La(3+) or a 
      Ca(2+)-free medium, inhibited ATP-dependent stress activated Ca(2+) (SAC) influx, 
      but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was 
      diminished by two PI-PLC inhibitors and an IP(3) receptor antagonist. These 
      results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that 
      phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP(3)/Ca(2+)(i) release 
      but independent of SAC influx in these cells, differently to other cell types.
FAU - Scodelaro Bilbao, Paola
AU  - Scodelaro Bilbao P
AD  - Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur, 
      San Juan 670, (B8000ICN) Bahia Blanca, Argentina.
FAU - Boland, Ricardo
AU  - Boland R
FAU - Russo de Boland, Ana
AU  - Russo de Boland A
FAU - Santillan, Graciela
AU  - Santillan G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070731
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/*administration & dosage
MH  - Breast Neoplasms/*metabolism
MH  - Calcium/*metabolism
MH  - Cell Line, Tumor
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects
MH  - Metabolic Clearance Rate/drug effects
MH  - Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2007/08/28 09:00
MHDA- 2007/11/14 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/05/04 00:00 [received]
PHST- 2007/07/18 00:00 [revised]
PHST- 2007/07/19 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2007/11/14 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - S0003-9861(07)00368-2 [pii]
AID - 10.1016/j.abb.2007.07.012 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2007 Oct 1;466(1):15-23. doi: 10.1016/j.abb.2007.07.012. 
      Epub 2007 Jul 31.