PMID- 17720196
OWN - NLM
STAT- MEDLINE
DCOM- 20080610
LR  - 20080505
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 147
IP  - 1
DP  - 2008 Jun 1
TI  - Up-regulation of interleukin-10 mRNA expression in peripheral leukocytes predicts 
      poor outcome and diminished human leukocyte antigen-DR expression on monocytes in 
      septic patients.
PG  - 1-8
AB  - BACKGROUND: None of the parameters can predict the deterioration of sepsis early 
      enough. A new predictor allowing earlier detection of changes in septic patients' 
      condition is indispensable. METHODS: We examined whether cytokine-related mRNA 
      expression in peripheral leukocytes, cytokine blood level, and monocyte human 
      leukocyte antigen (HLA)-DR expression rate could be useful predictors of 
      deterioration of sepsis. Twenty septic patients were enrolled. mRNA expression 
      levels were quantified using real-time reverse transcriptase-polymerase chain 
      reaction and assessed by percent changes during the first 24 h. Cytokine blood 
      levels on admission and day 3 and monocyte HLA-DR expression rate on day 3 were 
      also measured. Correlations between each of these parameters, and between these 
      parameters and outcome were analyzed. RESULTS: Among seven kinds of cytokines 
      studied, only IL-10 mRNA level showed significant difference between survivors 
      and non-survivors (P < 0.05). Mortality rate of interleukin (IL)-10 mRNA level 
      up-regulated group was significantly higher than that of IL-10 mRNA level 
      down-regulated group (P < 0.05). HLA-DR expression rate in non-survivors were 
      significantly lower than that in survivors (P < 0.05). Although IL-10 blood level 
      on day 3 significantly correlated with HLA-DR expression on day 3(r = 0.54, P < 
      0.05), IL-10 blood level on admission did not correlate with it. Contrarily, the 
      degree of up-regulation of IL-10 mRNA expression during the first 24 h 
      significantly correlated with the degree of diminished HLA-DR expression on day 3 
      (r = 0.78, P < 0.001). CONCLUSIONS: Up-regulated IL-10 mRNA expression and 
      diminished HLA-DR expression could be indicators of poor outcome. Furthermore, 
      IL-10 mRNA expression measurement could predict the onset of immunoparalysis 
      indicated by diminished HLA-DR expression earlier than IL-10 blood level 
      measurement, leading to earlier commencement of the treatment.
FAU - Abe, Ryuzo
AU  - Abe R
AD  - Department of Emergency and Critical Care Medicine, Graduate School of Medicine, 
      Chiba University, Chiba, Japan. ryuzo@pf6.so-net.ne.jp
FAU - Hirasawa, Hiroyuki
AU  - Hirasawa H
FAU - Oda, Shigeto
AU  - Oda S
FAU - Sadahiro, Tomohito
AU  - Sadahiro T
FAU - Nakamura, Masataka
AU  - Nakamura M
FAU - Watanabe, Eizo
AU  - Watanabe E
FAU - Nakada, Taka-Aki
AU  - Nakada TA
FAU - Hatano, Masahiko
AU  - Hatano M
FAU - Tokuhisa, Takeshi
AU  - Tokuhisa T
LA  - eng
PT  - Journal Article
DEP - 20070820
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Gene Expression Regulation
MH  - HLA-DR Antigens/*blood
MH  - Humans
MH  - Interleukin-10/blood/*genetics
MH  - Interleukin-6/blood
MH  - Leukocytes/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology
MH  - RNA, Messenger/*blood
MH  - Sepsis/*immunology
MH  - Up-Regulation
EDAT- 2007/08/28 09:00
MHDA- 2008/06/11 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/03/13 00:00 [received]
PHST- 2007/07/03 00:00 [revised]
PHST- 2007/07/09 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2008/06/11 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - S0022-4804(07)00471-4 [pii]
AID - 10.1016/j.jss.2007.07.009 [doi]
PST - ppublish
SO  - J Surg Res. 2008 Jun 1;147(1):1-8. doi: 10.1016/j.jss.2007.07.009. Epub 2007 Aug 
      20.