PMID- 17720472
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20131121
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 23
IP  - 4
DP  - 2007 Nov 30
TI  - Successively amplified electrochemical immunoassay based on biocatalytic 
      deposition of silver nanoparticles and silver enhancement.
PG  - 485-91
AB  - A successively signal-amplified electrochemical immunoassay has been reported on 
      the basis of the biocatalytic deposition of silver nanoparticles with their 
      subsequent enlargement by nanoparticle-promoted catalytic precipitation of silver 
      from the silver-enhancer solution. The immunoassay was carried out based on a 
      heterogeneous sandwich procedure using polystyrene microwells to immobilize 
      antibody. After all the processes comprising the formation of immunocomplex, 
      biocatalytic deposition of silver nanoparticles and following silver enhancement 
      were completed, the silver on polystyrene microwells was dissolved and quantified 
      by anodic stripping voltammetry (ASV). The effect of relevant experimental 
      conditions, including the concentration of ascorbic acid 2-phosphate (AA-p) 
      substrate and Ag(I) ions, the biocatalytic deposition time, and of crucial 
      importance, the silver enhancement time, were investigated and optimized. The 
      anodic stripping peak current was proportional to the concentration of human IgG 
      in a dynamic range of 0.1-10 ng ml(-1) with a detection limit of 0.03 ng ml(-1). 
      Scanning electron microscope (SEM) was applied to characterize the silver 
      nanoparticles before and after silver enhancement on the surface of polystyrene 
      microplates. By coupling the highly catalytic effect of enzyme and nanoparticles 
      to successively amplify the analytical signal, the sensitivity of immunoassay was 
      enhanced so dramatically that this approach would be a promising strategy to 
      achieve a lower detection limit for bioassays.
FAU - Chen, Zhao-Peng
AU  - Chen ZP
AD  - State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha 410082, China.
FAU - Peng, Zhao-Feng
AU  - Peng ZF
FAU - Luo, Yan
AU  - Luo Y
FAU - Qu, Bo
AU  - Qu B
FAU - Jiang, Jian-Hui
AU  - Jiang JH
FAU - Zhang, Xiao-Bing
AU  - Zhang XB
FAU - Shen, Guo-Li
AU  - Shen GL
FAU - Yu, Ru-Qin
AU  - Yu RQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070720
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Biosensing Techniques/methods
MH  - Catalysis
MH  - Electrochemistry/*methods
MH  - Immunoassay/methods
MH  - Nanoparticles/*chemistry
MH  - Nanotechnology/methods
MH  - Reproducibility of Results
MH  - Silver/*chemistry
EDAT- 2007/08/28 09:00
MHDA- 2008/02/27 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/02/11 00:00 [received]
PHST- 2007/05/29 00:00 [revised]
PHST- 2007/06/22 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - S0956-5663(07)00268-0 [pii]
AID - 10.1016/j.bios.2007.06.005 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2007 Nov 30;23(4):485-91. doi: 10.1016/j.bios.2007.06.005. 
      Epub 2007 Jul 20.