PMID- 17720554
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20101118
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 31
IP  - 12
DP  - 2007 Dec
TI  - Effect of neurotrophins on differentiation, calcification and proliferation in 
      cultures of human pulp cells.
PG  - 1462-9
AB  - Neurotrophins (NTs) are expressed during tooth development. However, little is 
      known about a role of NTs in differentiation of pulp cells into mineralizing 
      cells. In this study, mRNA expressions of hard tissue-related proteins, 
      calcification and proliferation are examined in cultures of human pulp (HP) 
      cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), 
      neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin 
      sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone 
      morphogenetic protein-2 and mineral deposition in cultures of HP cells. The 
      increased levels and manners varied, depending on the concentrations of NTs and 
      hard-tissue related protein tested. On the other hand, only NGF significantly 
      stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs 
      characteristically regulate hard-tissue related protein expression, calcification 
      and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.
FAU - Mizuno, Noriyoshi
AU  - Mizuno N
AD  - Department of Periodontal Medicine, Division of Frontier Medical Science, 
      Programs for Biomedical Research, Hiroshima University Graduate School of 
      Biomedical Sciences, 1-2-3, Kasumi, Hiroshima 734-8553, Japan. 
      mizuno@hiroshima-u.ac.jp
FAU - Shiba, Hideki
AU  - Shiba H
FAU - Xu, Wan-peng
AU  - Xu WP
FAU - Inui, Takafumi
AU  - Inui T
FAU - Fujita, Tsuyoshi
AU  - Fujita T
FAU - Kajiya, Mikihito
AU  - Kajiya M
FAU - Takeda, Katsuhiro
AU  - Takeda K
FAU - Hasegawa, Naohiko
AU  - Hasegawa N
FAU - Kawaguchi, Hiroyuki
AU  - Kawaguchi H
FAU - Kurihara, Hidemi
AU  - Kurihara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070717
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Collagen Type I)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (SPP1 protein, human)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (dentin sialophosphoprotein)
RN  - 106441-73-0 (Osteopontin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Calcification, Physiologic/physiology
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - Dental Pulp/*cytology/*metabolism
MH  - Extracellular Matrix Proteins/metabolism
MH  - Humans
MH  - Nerve Growth Factors/*metabolism
MH  - Odontogenesis/*physiology
MH  - Osteopontin/genetics/metabolism
MH  - Phosphoproteins
MH  - RNA, Messenger/metabolism
MH  - Receptors, Nerve Growth Factor/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sialoglycoproteins
MH  - Transforming Growth Factor beta/metabolism
EDAT- 2007/08/28 09:00
MHDA- 2008/01/11 09:00
CRDT- 2007/08/28 09:00
PHST- 2006/06/28 00:00 [received]
PHST- 2007/03/30 00:00 [revised]
PHST- 2007/06/26 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - S1065-6995(07)00184-9 [pii]
AID - 10.1016/j.cellbi.2007.06.012 [doi]
PST - ppublish
SO  - Cell Biol Int. 2007 Dec;31(12):1462-9. doi: 10.1016/j.cellbi.2007.06.012. Epub 
      2007 Jul 17.