PMID- 17721724
OWN - NLM
STAT- MEDLINE
DCOM- 20080122
LR  - 20181113
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 43
IP  - 7
DP  - 2007 Jul-Aug
TI  - Observations of Forsythia koreana methanol extract on mast cell-mediated allergic 
      reactions in experimental models.
PG  - 215-21
AB  - To explore effects of Forsythia koreana methanol extract (FKME) on mast 
      cell-mediated allergic and inflammatory properties, the effect of FKME was 
      evaluated on compound 48/80-induced systemic anaphylaxis, ear swelling, and 
      anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous 
      anaphylaxis (PCA). In addition, the effect of FKME was investigated on the 
      histamine release from rat peritoneal mast cells (RPMCs) stimulated by compound 
      48/80, which promotes histamine release. The human mast cell line HMC-1 was 
      stimulated by phorbol 12-myristate 13-acetate plus calcium ionophore A23187. 
      Activated HMC-1 can produce several proinflammatory and chemotactic cytokines 
      such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, and IL-8. 
      Cytokine levels in the culture supernatant were measured by an enzyme-linked 
      immunosorbent assay. Cytotoxicity by FKME was determined by a 
      3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium bromide (MTT) assay. FKME 
      inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in 
      mice. When 1 g/kg FKME was pretreated or posttreated with mice, compound 
      48/80-induced mice morality was 50 and 66.7%, respectively. One gram per kilogram 
      of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 
      by 29.75%. A PCA reaction was inhibited by 17.9%. In an in vitro model, FKME (1 
      mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-alpha, IL-6, 
      and IL-8 production from HMC-1 cells by 71.16% (P < 0.001), 86.72% (P < 0.001), 
      and 44.6%, respectively. However, FKME had no cytotoxic effects on cell 
      viability. In conclusion, FKME inhibited not only systemic anaphylaxis and ear 
      swelling induced by compound 48/80 but also inhibited a PCA reaction induced by 
      anti-DNP IgE in vivo. Treatment with FKME showed significant inhibitory effects 
      on histamine, TNF-alpha, IL-6, and IL-8 release from mast cells.
FAU - Choi, In-Young
AU  - Choi IY
AD  - Department of Pharmacology, College of Oriental Medicine, Institute of Oriental 
      Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South 
      Korea.
FAU - Moon, Phil-Dong
AU  - Moon PD
FAU - Koo, Hyun-Na
AU  - Koo HN
FAU - Myung, Noh-Yil
AU  - Myung NY
FAU - Kim, Su-Jin
AU  - Kim SJ
FAU - Lee, Ji-Hyun
AU  - Lee JH
FAU - Han, Se-Hee
AU  - Han SH
FAU - Moon, Goo
AU  - Moon G
FAU - Seo, Sung-Yum
AU  - Seo SY
FAU - Sung, Hyun-Jea
AU  - Sung HJ
FAU - Park, Rae-Kil
AU  - Park RK
FAU - Jeong, Hyun-Ja
AU  - Jeong HJ
FAU - Um, Jae-Young
AU  - Um JY
FAU - Kim, Hyung-Min
AU  - Kim HM
FAU - Hong, Seung-Heon
AU  - Hong SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070825
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Anaphylaxis/chemically induced/immunology
MH  - Animals
MH  - Cell Line
MH  - *Forsythia/chemistry/immunology
MH  - Histamine Release
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Mast Cells/cytology/*immunology
MH  - Methanol/*chemistry
MH  - Mice
MH  - Passive Cutaneous Anaphylaxis/immunology
MH  - Plant Extracts/chemistry/*immunology
MH  - Rats
MH  - Rats, Wistar
MH  - p-Methoxy-N-methylphenethylamine/immunology
EDAT- 2007/08/28 09:00
MHDA- 2008/01/23 09:00
CRDT- 2007/08/28 09:00
PHST- 2007/04/15 00:00 [received]
PHST- 2007/05/15 00:00 [accepted]
PHST- 2007/08/28 09:00 [pubmed]
PHST- 2008/01/23 09:00 [medline]
PHST- 2007/08/28 09:00 [entrez]
AID - 10.1007/s11626-007-9040-6 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2007 Jul-Aug;43(7):215-21. doi: 
      10.1007/s11626-007-9040-6. Epub 2007 Aug 25.