PMID- 17723411
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070829
LR  - 20070828
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 571
IP  - 1
DP  - 2006 Jun 30
TI  - Optimization of LC-MS/MS using triple quadrupole mass analyzer for the 
      simultaneous analysis of carbosulfan and its main metabolites in oranges.
PG  - 1-11
AB  - This paper describes an analytical method involving a simple solvent extraction 
      for the simultaneous liquid chromatography coupled to quadrupole tandem mass 
      spectrometry (LC-MS/MS) determination of carbosulfan, its most toxic 
      metabolite--carbofuran--, and its other main metabolites--3-hydroxycarbofuran, 
      3-ketocarbofuran, 3-hydroxy-7-phenolcarbofuran, 3-keto-7-phenolcarbofuran, 
      7-phenolcarbofuran and dibutylamine--in oranges. Chromatography was performed on 
      a Zorbax Bonus-RP (150 mm x 2.1 mm, 5 microm). The mobile phase was a ternary 
      gradient water-methanol-acetonitrile with 1.0 mM ammonium acetate at flow rate of 
      0.2 ml min(-1). The LC separation and MS/MS optimization were studied to select 
      the most appropriate operating conditions. The method developed has also been 
      validated. The limits of quantification (LOQs) were from 1 microg kg(-1) for 
      carbofuran to 10 microg kg(-1) for 3-keto-7-phenolcarbofuran. Extracts spiked 
      with carbosulfan and its metabolites, at LOQ level, yielded average recoveries in 
      the range 60-94%, with relative standard deviations (R.S.D.s) less than 15%. 
      Calibration curves for carbosulfan and its metabolites (range LOQ-1000LOQ) were 
      linear, with coefficients of correlations better than 0.990. The method was 
      successfully applied to establish the primary degradation products in oranges 
      treated with carbosulfan. The LC-MS/MS method developed is simple, rapid, and 
      suitable for the quantification and confirmation of carbosulfan and seven of its 
      main metabolites in orange at levels lower than 10 microg kg(-1).
FAU - Soler, Carla
AU  - Soler C
AD  - Laboratori de Bromatologia i Toxicologia, Facultat de Farmacia, Universitat de 
      Valencia, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain. 
      carla.soler@uv.es
FAU - Hamilton, Brett
AU  - Hamilton B
FAU - Furey, Ambrose
AU  - Furey A
FAU - James, Kevin J
AU  - James KJ
FAU - Manes, Jordi
AU  - Manes J
FAU - Pico, Yolanda
AU  - Pico Y
LA  - eng
PT  - Journal Article
DEP - 20060427
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
EDAT- 2007/08/29 09:00
MHDA- 2007/08/29 09:01
CRDT- 2007/08/29 09:00
PHST- 2006/03/18 00:00 [received]
PHST- 2006/04/11 00:00 [revised]
PHST- 2006/04/13 00:00 [accepted]
PHST- 2007/08/29 09:00 [pubmed]
PHST- 2007/08/29 09:01 [medline]
PHST- 2007/08/29 09:00 [entrez]
AID - S0003-2670(06)00868-3 [pii]
AID - 10.1016/j.aca.2006.04.033 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2006 Jun 30;571(1):1-11. doi: 10.1016/j.aca.2006.04.033. Epub 
      2006 Apr 27.