PMID- 17723460
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070829
LR  - 20070828
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 572
IP  - 1
DP  - 2006 Jul 14
TI  - Analysis of fatty acids in lung tissues using gas chromatography-mass 
      spectrometry preceded by derivatization-solid-phase microextraction with a novel 
      fiber.
PG  - 47-54
AB  - In this article, a laboratory-made sol-gel derived fiber with butyl 
      methacrylate/hydroxy-terminated silicone oil (BMA/OH-TSO) coating was first used 
      for headspace solid-phase microextraction (HS-SPME) of medium and long chain 
      fatty acids after derivatization and applied to the analysis of fatty acids in 
      lung tissues by coupling to gas chromatography-mass spectrometry (GC-MS). The 
      experimental parameters for derivatization, HS-SPME and desorption were 
      optimized. Fatty acids in cancerous lung tissues from five patients with lung 
      cancer were determined under the optimized conditions. Normal lung tissues from 
      the same five patients were used as controls. This fiber showed higher extraction 
      efficiency for fatty acids after derivatization when compared with commercial 
      polydimethylsiloxane (PDMS) and polydimethylsiloxane-divinylbenzene (PDMS/DVB) 
      fibers due to the three-dimensional network in the coating. The method presented 
      in this paper showed satisfactory precision, accuracy, linearity and limits of 
      detection (LODs). The relative standard deviation values were below 13.3% (n=5) 
      and the recoveries obtained ranged from 76.35% to 107.0%. The results obtained 
      using the SPME method were also compared with those got by using liquid-liquid 
      extraction (LLE) technique. It was found that the sensitivity could be enhanced 
      by the SPME method. The analysis of the cancerous lung tissues and normal 
      controls from five patients with lung cancer indicated that the main components 
      of lung tissue were palmitic acid (C16:0), stearic acid (C18:0) and lignoceric 
      acid (C24:0). A comparison between the levels of the fatty acids in cancerous 
      lung tissues and normal controls from the same a patient with lung cancer shows 
      that most of the saturated fatty acids showed higher levels in cancerous lung 
      tissues, while unsaturated fatty acids showed higher levels in normal controls on 
      the whole.
FAU - Cha, Dongmei
AU  - Cha D
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, China.
FAU - Liu, Mingming
AU  - Liu M
FAU - Zeng, Zhaorui
AU  - Zeng Z
FAU - Cheng, Dong'e
AU  - Cheng D
FAU - Zhan, Guoqing
AU  - Zhan G
LA  - eng
PT  - Journal Article
DEP - 20060510
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
EDAT- 2007/08/29 09:00
MHDA- 2007/08/29 09:01
CRDT- 2007/08/29 09:00
PHST- 2006/01/20 00:00 [received]
PHST- 2006/04/30 00:00 [revised]
PHST- 2006/05/04 00:00 [accepted]
PHST- 2007/08/29 09:00 [pubmed]
PHST- 2007/08/29 09:01 [medline]
PHST- 2007/08/29 09:00 [entrez]
AID - S0003-2670(06)00948-2 [pii]
AID - 10.1016/j.aca.2006.05.014 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2006 Jul 14;572(1):47-54. doi: 10.1016/j.aca.2006.05.014. Epub 
      2006 May 10.