PMID- 17723485
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070830
LR  - 20070828
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 572
IP  - 2
DP  - 2006 Jul 21
TI  - Application of SiO2-poly(dimethylsiloxane) hybrid material in the fabrication of 
      amperometric biosensor.
PG  - 248-52
AB  - Silica xerogel was widely used in the development of biosensors by coupling the 
      desired biological components to various transducers. Unfortunately, the 
      application of xerogels is limited due to their poor mechanical properties and 
      poor structural maintenance of entrapped biomaterials. In this study, the 
      availability of poly(dimethylsiloxane) (PDMS)-modified silica sol-gel (TEOS/PDMS 
      Ormosil) glass in the immobilization of enzymes and its application in the 
      fabrication of amperometric biosensor was investigated. We demonstrated that most 
      of activity of encapsulated horseradish peroxidase can be preserved in 
      PDMS-modified SiO2 glass compared with conventional silica xerogel. The 
      synthesized monoliths exhibited high transparency and crack-free. The enzyme 
      electrode based on glucose oxidase encapsulated TEOS/PDMS Ormosil glass on Pd 
      electrode was fabricated and characterized electrochemically. The characteristics 
      of the biosensor were studied by cyclic voltammetry and chronoamperometry. The 
      biosensor exhibited a series of good properties: high sensitivity (1.63 microA 
      mM(-1) analyte), short response time and high reproducibility. The results 
      indicate that TEOS/PDMS Ormosil glass has great potential to the immobilization 
      of biomaterials as well as the fabrication of biosensors.
FAU - Ho, Wei-Jen
AU  - Ho WJ
AD  - Department of Biological Science and Technology, National Chiao Tung University, 
      Hsinchu, Taiwan 300, ROC.
FAU - Yuan, Chiun-Jye
AU  - Yuan CJ
FAU - Reiko, Ohara
AU  - Reiko O
LA  - eng
PT  - Journal Article
DEP - 20060519
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
EDAT- 2007/08/29 09:00
MHDA- 2007/08/29 09:01
CRDT- 2007/08/29 09:00
PHST- 2005/11/28 00:00 [received]
PHST- 2006/05/01 00:00 [revised]
PHST- 2006/05/06 00:00 [accepted]
PHST- 2007/08/29 09:00 [pubmed]
PHST- 2007/08/29 09:01 [medline]
PHST- 2007/08/29 09:00 [entrez]
AID - S0003-2670(06)00989-5 [pii]
AID - 10.1016/j.aca.2006.05.022 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2006 Jul 21;572(2):248-52. doi: 10.1016/j.aca.2006.05.022. Epub 
      2006 May 19.