PMID- 1774958
OWN - NLM
STAT- MEDLINE
DCOM- 19920304
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 5
IP  - 12
DP  - 1991 Dec
TI  - Diagnostic and prognostic values of in vitro culture growth patterns of marrow 
      granulocyte-macrophage progenitors in patients with myelodysplastic syndrome.
PG  - 1092-8
AB  - The in vitro culture growth of marrow granulocyte-macrophage progenitors (CFU-GM 
      assay) was studied in 102 consecutive patients with newly diagnosed primary 
      myelodysplastic syndrome (MDS) to determine its diagnostic utility and prognostic 
      value. There were 18 patients with refractory anemia (RA), eight RA with 
      ringed-sideroblast (RARS), 30 RA with excess of blasts (RAEB), 18 chronic 
      myelomonocytic leukemia (CMML), and 28 RAEB in transformation (RAEB-T). Patients 
      with MDS had a significantly lower number of GM colonies and a significantly 
      higher cluster to colony ratio than those of normal controls and patients with 
      cytopenias of other causes. Six in vitro growth patterns were observed; 85% of 
      patients with MDS showed various abnormal growth patterns, and 42% of all MDS 
      patients exhibited a leukemic growth pattern at diagnosis. None of the 40 
      patients with cytopenias of other causes had a leukemic type growth. A leukemic 
      growth pattern was rarely observed in patients with RA and RARS (4%), but was 
      common in other subgroups (57%). The distribution of various growth patterns was 
      not statistically different among patients with RAEB, CMML, and RAEB-T. 
      Thirty-six patients developed acute leukemia during the follow-up period. The MDS 
      patients with leukemic type growth were at increased risk of rapid progression to 
      acute leukemia, and they also had a shorter survival time than patients with a 
      non-leukemic pattern. These results showed that simply scoring the number of 
      CFU-GM is of limited value for the diagnosis and the prediction of prognosis of 
      MDS, whereas the in vitro marrow culture growth pattern is of prognostic 
      significance independently of the FAB classification. It is concluded that the in 
      vitro growth pattern of marrow CFU-GM is helpful in diagnosing patients with MDS 
      as well as in predicting their clinical outcome.
FAU - Shih, L Y
AU  - Shih LY
AD  - Department of Internal Medicine, Chang Gung Memorial Hospital, Chang Gung Medical 
      College, Taipei, Taiwan, Republic of China.
FAU - Chiu, W F
AU  - Chiu WF
FAU - Lee, C T
AU  - Lee CT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adult
MH  - Bone Marrow/*pathology
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Granulocytes/*cytology
MH  - Humans
MH  - Leukemia/pathology
MH  - Macrophages/*cytology
MH  - Myelodysplastic Syndromes/*diagnosis
MH  - Preleukemia/diagnosis
MH  - Prognosis
MH  - Prospective Studies
MH  - Survival Analysis
EDAT- 1991/12/01 00:00
MHDA- 1991/12/01 00:01
CRDT- 1991/12/01 00:00
PHST- 1991/12/01 00:00 [pubmed]
PHST- 1991/12/01 00:01 [medline]
PHST- 1991/12/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1991 Dec;5(12):1092-8.