PMID- 17762522
OWN - NLM
STAT- MEDLINE
DCOM- 20071023
LR  - 20090707
IS  - 0955-8810 (Print)
IS  - 0955-8810 (Linking)
VI  - 18
IP  - 5-6
DP  - 2007 Sep
TI  - Bidirectional changes in water-maze learning following recombinant 
      adenovirus-associated viral vector (rAAV)-mediated brain-derived neurotrophic 
      factor expression in the rat hippocampus.
PG  - 533-47
AB  - Alterations in hippocampal brain-derived neurotrophic factor (BDNF) expression 
      have been implicated in the pathogenesis of emotional and cognitive dysfunction. 
      Here, we induced BDNF overexpression in the rat hippocampus using recombinant 
      adenovirus-associated viral (rAAV) vectors, and studied its long-term (2 months 
      postinduction) effects on anxiety-related behaviour, exploration in the open 
      field, and spatial learning in the water maze. Although the treatment 
      successfully led to substantial elevation of hippocampal BDNF levels, its effect 
      on spatial learning was bidirectional: a subset of rAAV-induced 
      BDNF-overexpressing rats performed well above control level, whereas the rest 
      were clearly impaired. This behavioural distinction corresponded to two markedly 
      different levels of BDNF overexpression. The increase in dorsal hippocampal BDNF 
      content achieved in the 'water-maze-impaired' subgroup was twice that attained in 
      the 'water-maze-improved' rats. Although neither subgroup of rAAV-induced 
      BDNF-overexpressing rats differed from controls in the open field, the 
      'water-maze-impaired' subgroup also showed a significant anxiolytic effect. Our 
      results suggest that hippocampal BDNF elevation significantly affects cognitive 
      and emotional behaviours, but the direction and magnitude of the effects 
      critically depend on the precise levels of overexpression. This factor must be 
      taken into account in future studies examining the functional consequences of 
      hippocampal BDNF overexpression.
FAU - Pietropaolo, Susanna
AU  - Pietropaolo S
AD  - Laboratory of Behavioural Neurobiology, ETH Zurich, Switzerland.
FAU - Paterna, Jean-Charles
AU  - Paterna JC
FAU - Bueler, Hansruedi
AU  - Bueler H
FAU - Feldon, Joram
AU  - Feldon J
FAU - Yee, Benjamin K
AU  - Yee BK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Behav Pharmacol
JT  - Behavioural pharmacology
JID - 9013016
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adenoviridae
MH  - Animals
MH  - Anxiety/*physiopathology
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cognition Disorders/*physiopathology
MH  - Emotions/physiology
MH  - Exploratory Behavior/physiology
MH  - Gene Expression Regulation
MH  - Genetic Vectors
MH  - Hippocampus/metabolism
MH  - Male
MH  - Maze Learning/*physiology
MH  - Memory/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Spatial Behavior/physiology
EDAT- 2007/09/01 09:00
MHDA- 2007/10/24 09:00
CRDT- 2007/09/01 09:00
PHST- 2007/09/01 09:00 [pubmed]
PHST- 2007/10/24 09:00 [medline]
PHST- 2007/09/01 09:00 [entrez]
AID - 00008877-200709000-00021 [pii]
AID - 10.1097/FBP.0b013e3282da0bf6 [doi]
PST - ppublish
SO  - Behav Pharmacol. 2007 Sep;18(5-6):533-47. doi: 10.1097/FBP.0b013e3282da0bf6.