PMID- 17762938
OWN - NLM
STAT- MEDLINE
DCOM- 20080125
LR  - 20181113
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 128
IP  - 4
DP  - 2007 Oct
TI  - Synchronized reconstitution of muscle fibers, peripheral nerves and blood vessels 
      by murine skeletal muscle-derived CD34(-)/45 (-) cells.
PG  - 349-60
AB  - In order to establish the practical isolation and usage of skeletal 
      muscle-derived stem cells (MDSCs), we determined reconstitution capacity of 
      CD34(-)/CD45(-) (Sk-DN) cells as a candidate somatic stem cell source for 
      transplantation. Sk-DN cells were enzymatically isolated from GFP transgenic mice 
      (C57/BL6N) skeletal muscle and sorted using fluorescence activated cell sorting 
      (FACS), and expanded by collagen gel-based cell culture with bFGF and EGF. The 
      number of Sk-DN cells was small after sorting (2-8 x 10(4)); however, the number 
      increased 10-20 fold (2-16 x 10(5)) after 6 days of expansion culture, and the 
      cells maintained immature state and multipotency, expressing mRNAs for mesodermal 
      and ectodermal cell lineages. Transplantation of expanded Sk-DN cells into the 
      severe muscle damage model (C57/BL6N wild-type) resulted in the synchronized 
      reconstitution of blood vessels, peripheral nerves and muscle fibers following 
      significant recovery of total muscle mass (57%) and contractile function (55%), 
      whereas the non-cell-transplanted control group showed around 20% recovery in 
      both factors. These reconstitution capacities were supported by the intrinsic 
      plasticity of Sk-DN cells that can differentiate into muscular (skeletal muscle), 
      vascular (pericyte, endothelial cell and smooth muscle) and peripheral nerve 
      (Schwann cells and perineurium) cell lineages that was revealed by 
      transplantation to non-muscle tissue (beneath renal capsule) and fluorescence in 
      situ hybridization (FISH) analysis.
FAU - Tamaki, Tetsuro
AU  - Tamaki T
AD  - Muscle Physiology and Cell Biology Unit, Tokai University School of Medicine, 
      Shimokasuya-143, Isehara, Kanagawa, 259-1143, Japan. tamaki@is.icc.u-tokai.ac.jp
FAU - Okada, Yoshinori
AU  - Okada Y
FAU - Uchiyama, Yoshiyasu
AU  - Uchiyama Y
FAU - Tono, Kayoko
AU  - Tono K
FAU - Masuda, Maki
AU  - Masuda M
FAU - Wada, Mika
AU  - Wada M
FAU - Hoshi, Akio
AU  - Hoshi A
FAU - Akatsuka, Akira
AU  - Akatsuka A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070829
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Antigens, CD34)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocyte Common Antigens/*deficiency/genetics/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Immunoelectron
MH  - Muscle Development
MH  - Muscle Fibers, Skeletal/*metabolism/ultrastructure
MH  - Muscle, Skeletal/*blood supply/*metabolism
MH  - Peripheral Nerves/*metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
EDAT- 2007/09/01 09:00
MHDA- 2008/01/26 09:00
CRDT- 2007/09/01 09:00
PHST- 2007/08/14 00:00 [accepted]
PHST- 2007/09/01 09:00 [pubmed]
PHST- 2008/01/26 09:00 [medline]
PHST- 2007/09/01 09:00 [entrez]
AID - 10.1007/s00418-007-0331-5 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2007 Oct;128(4):349-60. doi: 10.1007/s00418-007-0331-5. Epub 
      2007 Aug 29.