PMID- 17764512
OWN - NLM
STAT- MEDLINE
DCOM- 20080606
LR  - 20171116
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 47
IP  - 12
DP  - 2007 Dec
TI  - Monocyte enrichment from leukapheresis products by using the Elutra cell 
      separator.
PG  - 2290-6
AB  - BACKGROUND: Dendritic cells (DCs), used in clinical trials for cancer 
      immunotherapy, require processing on an expanded scale to conform to current good 
      manufacturing practice guidelines. This study evaluated a large-scale monocyte 
      enrichment procedure with a commercially available cell separator (Elutra, Gambro 
      BCT) and analyzed the capacity of enriched monocytes to differentiate into DCs. 
      STUDY DESIGN AND METHODS: Mononuclear cells were collected in two patients with 
      malignant melanoma and seven healthy donors by leukapheresis. 
      Continuous-counterflow elutriation with the Elutra was performed to enrich and 
      purify monocytes from leukapheresis products. Purity and recovery of enriched 
      monocytes were analyzed by flow cytometry. DCs were generated from the elutriated 
      monocytes and characterized by phenotypic surface marker and stimulatory capacity 
      in an allogeneic mixed lymphocyte reaction. RESULTS: In the leukapheresis 
      products, the total MNC count was 7.3 x 10(9) +/- 0.7 x 10(9) and the mean 
      percentage of CD14+ monocytes was 16.5 +/- 3.8 percent, which increased to 68.9 
      +/- 7.4 percent after elutriation with the Elutra. The mean monocyte recovery was 
      94.3 percent. Elutriated monocytes were successfully cultured into phenotypically 
      and functionally mature DCs. CONCLUSION: These results indicate that the Elutra 
      cell separator allows for fast and easy enrichment of monocytes within a closed 
      system. Furthermore, these monocytes can be differentiated into functionally 
      mature DCs. Compared to plastic adherence and immunomagnetic selection methods, 
      the elutriation procedure is inexpensive, efficient, and very effective.
FAU - Kim, Sinyoung
AU  - Kim S
AD  - Department of Laboratory Medicine, Yonsei Cell Therapy Center, Department of 
      Dermatology, Yonsei University College of Medicine, Seoul, Korea.
FAU - Kim, Hyun Ok
AU  - Kim HO
FAU - Baek, Eun-Jung
AU  - Baek EJ
FAU - Choi, Youjeong
AU  - Choi Y
FAU - Kim, Han-Soo
AU  - Kim HS
FAU - Lee, Min-Geul
AU  - Lee MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070830
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Lipopolysaccharide Receptors)
SB  - IM
MH  - Cell Separation/instrumentation/*methods
MH  - Dendritic Cells/*cytology/immunology
MH  - Humans
MH  - Leukapheresis/*methods
MH  - Leukocytes, Mononuclear/*cytology/immunology
MH  - Lipopolysaccharide Receptors/analysis
MH  - Reproducibility of Results
EDAT- 2007/09/04 09:00
MHDA- 2008/06/07 09:00
CRDT- 2007/09/04 09:00
PHST- 2007/09/04 09:00 [pubmed]
PHST- 2008/06/07 09:00 [medline]
PHST- 2007/09/04 09:00 [entrez]
AID - TRF01470 [pii]
AID - 10.1111/j.1537-2995.2007.01470.x [doi]
PST - ppublish
SO  - Transfusion. 2007 Dec;47(12):2290-6. doi: 10.1111/j.1537-2995.2007.01470.x. Epub 
      2007 Aug 30.