PMID- 17764660
OWN - NLM
STAT- MEDLINE
DCOM- 20071101
LR  - 20070911
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 362
IP  - 3
DP  - 2007 Oct 26
TI  - Expression profiles of BDNF splice variants in cultured DRG neurons stimulated 
      with NGF.
PG  - 682-8
AB  - Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the 
      dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth 
      factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. 
      Recently, it was reported that the BDNF gene has eight non-coding exons that are 
      transcribed independently into several splice variants. Expression of these 
      splice variants in DRG neurons stimulated with NGF has not been studied. We 
      examined changes in expression of BDNF splice variants in a rat model of 
      peripheral inflammation and in cultured DRG neurons exposed to NGF. Total BDNF 
      mRNA was increased by inflammation in vivo and by NGF in vitro. Among all splice 
      variants, exon 1-9 showed the greatest increase in expression in both 
      experiments. Our results indicate that exon 1-9 contributes to changes in total 
      BDNF levels and may play an important role in the acute response of DRG to NGF.
FAU - Matsuoka, Yoshikazu
AU  - Matsuoka Y
AD  - Department of Anesthesiology and Resuscitology, Okayama University Graduate 
      School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama City 
      700-8558, Japan.
FAU - Yokoyama, Masataka
AU  - Yokoyama M
FAU - Kobayashi, Hiroyuki
AU  - Kobayashi H
FAU - Omori, Megumi
AU  - Omori M
FAU - Itano, Yoshitaro
AU  - Itano Y
FAU - Morita, Kiyoshi
AU  - Morita K
FAU - Mori, Hiroki
AU  - Mori H
FAU - Nakanishi, Tohru
AU  - Nakanishi T
LA  - eng
PT  - Journal Article
DEP - 20070813
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Oligonucleotides)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/*genetics
MH  - Cells, Cultured
MH  - Exons
MH  - Ganglia, Spinal/*metabolism
MH  - *Gene Expression Regulation
MH  - Inflammation
MH  - Male
MH  - Nerve Growth Factor/*metabolism
MH  - Neurons/*metabolism
MH  - Oligonucleotides/chemistry
MH  - Pain
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2007/09/04 09:00
MHDA- 2007/11/02 09:00
CRDT- 2007/09/04 09:00
PHST- 2007/07/30 00:00 [received]
PHST- 2007/08/03 00:00 [accepted]
PHST- 2007/09/04 09:00 [pubmed]
PHST- 2007/11/02 09:00 [medline]
PHST- 2007/09/04 09:00 [entrez]
AID - S0006-291X(07)01738-X [pii]
AID - 10.1016/j.bbrc.2007.08.022 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Oct 26;362(3):682-8. doi: 
      10.1016/j.bbrc.2007.08.022. Epub 2007 Aug 13.