PMID- 17765576
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20071009
IS  - 1074-7427 (Print)
IS  - 1074-7427 (Linking)
VI  - 88
IP  - 4
DP  - 2007 Nov
TI  - Contextual learning induces an increase in the number of hippocampal CA1 neurons 
      expressing high levels of BDNF.
PG  - 409-15
AB  - We examined behaviorally induced expression of brain-derived neurotrophic factor 
      (BDNF) in area CA1 of the hippocampus. Sprague-Dawley rats were trained in a 
      contextual fear conditioning (CFC) task, sacrificed 4h later, and their brains 
      were processed for immunohistochemistry. We found distinctively high levels of 
      BDNF immunoreactivity in a small number ( approximately 1%) of CA1 neurons in 
      untrained animals. The number of these exceptional neurons, which are identified 
      as BDNF(++) in this study, was increased by up to approximately 3% after CFC. 
      This increase was blocked in the presence of a memory-impairing dose of a NMDA 
      receptor antagonist (MK801 0.3 mg/kg, i.p.) given 30 min prior to training. The 
      BDNF signal intensity in BDNF(++) neurons correlated with that of surrounding 
      glutamic acid decarboxylase (GAD) 65. This correlation between GAD65 and BDNF 
      signal intensities suggests that BDNF upregulation was associated with increased 
      signaling via inhibitory GABAergic synapses that would lessen further intervening 
      neuronal activity. Our observation that neurons which upregulate BDNF expression 
      following a learning experience are rich in GAD65-enriched afferent synapses 
      suggests that these neurons may have distinct roles in memory consolidation.
FAU - Chen, Jenru
AU  - Chen J
AD  - Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, 
      The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Kitanishi, Takuma
AU  - Kitanishi T
FAU - Ikeda, Takamitsu
AU  - Ikeda T
FAU - Matsuki, Norio
AU  - Matsuki N
FAU - Yamada, Maki K
AU  - Yamada MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070831
PL  - United States
TA  - Neurobiol Learn Mem
JT  - Neurobiology of learning and memory
JID - 9508166
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 2)
SB  - IM
MH  - Animals
MH  - Association Learning/*physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Conditioning, Classical/*physiology
MH  - Fear/physiology
MH  - Glutamate Decarboxylase/*metabolism
MH  - Hippocampus/cytology/*metabolism
MH  - Male
MH  - Neurons, Afferent/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synaptic Transmission
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2007/09/04 09:00
MHDA- 2008/01/09 09:00
CRDT- 2007/09/04 09:00
PHST- 2007/02/19 00:00 [received]
PHST- 2007/06/26 00:00 [revised]
PHST- 2007/07/15 00:00 [accepted]
PHST- 2007/09/04 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/09/04 09:00 [entrez]
AID - S1074-7427(07)00098-6 [pii]
AID - 10.1016/j.nlm.2007.07.009 [doi]
PST - ppublish
SO  - Neurobiol Learn Mem. 2007 Nov;88(4):409-15. doi: 10.1016/j.nlm.2007.07.009. Epub 
      2007 Aug 31.