PMID- 17767487
OWN - NLM
STAT- MEDLINE
DCOM- 20071128
LR  - 20211203
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 26
IP  - 5
DP  - 2007 Sep
TI  - Laser capture microdissection and microarray analysis of dividing neural 
      progenitor cells from the adult rat hippocampus.
PG  - 1079-90
AB  - Neural progenitor cells reside in the hippocampus of adult rodents and humans and 
      generate granule neurons throughout life. Knowledge about the molecular processes 
      regulating these neurogenic cells is fragmentary. In order to identify genes with 
      a role in the proliferation of adult neural progenitor cells, a protocol was 
      elaborated to enable the staining and isolation of such cells under 
      RNA-preserving conditions with a combination of immunohistochemistry and laser 
      capture microdissection. We increased proliferation of neural progenitor cells by 
      electroconvulsive treatment, one of the most effective antidepressant treatments, 
      and isolated Ki-67-positive cells using this new protocol. RNA amplification via 
      in vitro transcription and subsequent microarray analysis revealed over 100 genes 
      that were differentially expressed in neural progenitor cells due to 
      electroconvulsive treatment compared to untreated control animals. Some of these 
      genes have already been implicated in the functioning of neural progenitor cells 
      or have been induced by electroconvulsive treatment; these include brain-derived 
      neurotrophic factor (Bdnf), PDZ-binding kinase (Pbk) and abnormal spindle-like 
      microcephaly-associated (Aspm). In addition, genes were identified for which no 
      role in the proliferation of neurogenic progenitors has been described so far, 
      such as enhancer of zeste homolog 2 (Ezh2).
FAU - Gurok, Ulf
AU  - Gurok U
AD  - Neuroscience Discovery Research, Abbott, Knollstrasse, 67061 Ludwigshafen, 
      Germany. ulf.gurok@abbott.com
FAU - Loebbert, Ralf W
AU  - Loebbert RW
FAU - Meyer, Axel H
AU  - Meyer AH
FAU - Mueller, Reinhold
AU  - Mueller R
FAU - Schoemaker, Hans
AU  - Schoemaker H
FAU - Gross, Gerhard
AU  - Gross G
FAU - Behl, Berthold
AU  - Behl B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 2.7.12.2 (PDZ-binding kinase)
SB  - IM
MH  - Adult Stem Cells/*physiology/radiation effects
MH  - Animals
MH  - Cell Count
MH  - *Cell Proliferation/radiation effects
MH  - Electroshock/methods
MH  - Gene Expression Regulation/radiation effects
MH  - Hippocampus/*cytology
MH  - Ki-67 Antigen/metabolism
MH  - Lasers
MH  - Microarray Analysis/methods
MH  - Microdissection/methods
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Neurons/*physiology/radiation effects
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
EDAT- 2007/09/05 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/09/05 09:00
PHST- 2007/09/05 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/09/05 09:00 [entrez]
AID - EJN5734 [pii]
AID - 10.1111/j.1460-9568.2007.05734.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Sep;26(5):1079-90. doi: 10.1111/j.1460-9568.2007.05734.x.