PMID- 17768708
OWN - NLM
STAT- MEDLINE
DCOM- 20080424
LR  - 20071126
IS  - 1522-7235 (Print)
IS  - 1522-7235 (Linking)
VI  - 22
IP  - 6
DP  - 2007 Nov-Dec
TI  - Fluorescence quenching of serum albumin by rifamycin antibiotics and their 
      analytical application.
PG  - 559-66
AB  - In neutral medium, rifamycin antibiotics such as rifapentin (RFPT), rifampicin 
      (RFP), rifandin (RFD) and rifamycin SV (RFSV) can bind with human serum albumin 
      (HSA) and bovine serum albumin (BSA) to form complexes, resulting in the 
      quenching of the intrinsic fluorescence (lambda(ex)/lambda(em) = 285/355 nm) of 
      the BSA and HSA. The quenching intensity (DeltaF) is directly proportional to the 
      concentration of the rifamycin antibiotics. Therefore, a new analytical method 
      was established to determine trace rifamycin antibiotics. The method had fairly 
      high sensitivity and the detecting limits (3sigma) for RFPT, RFP, RFD and RFSV 
      were 0.85, 0.98, 1.83, 1.89 ng/mL, respectively, for the HSA system and 0.76, 
      0.89, 1.55, 1.77 ng/mL, respectively, for the BSA system. All relative standard 
      deviations (RSDs) were <3.8%. In this work, the characteristics of the 
      fluorescence spectra were studied and the optimum reaction conditions and 
      influencing factors were investigated. The influence of coexisting substances was 
      tested and the results showed that the method had good selectivity and could be 
      applied to determine trace rifamycin antibiotics in medicine capsules and urine 
      samples. Taking the RFSV-serum albumin system as an example, the reaction 
      mechanisms, such as binding constants, binding sites, binding distance and the 
      type of fluorescence quenching, were investigated.
CI  - Copyright (c) 2007 John Wiley & Sons, Ltd.
FAU - Yang, Ji-Dong
AU  - Yang JD
AD  - School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 
      People's Republic of China.
FAU - Deng, Shi-Xing
AU  - Deng SX
FAU - Liu, Zhong-Fang
AU  - Liu ZF
FAU - Kong, Ling
AU  - Kong L
FAU - Liu, Shao-Pu
AU  - Liu SP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Rifamycins)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/chemistry/*metabolism
MH  - Binding Sites
MH  - Cattle
MH  - Energy Transfer
MH  - Humans
MH  - Rifamycins/*analysis/chemistry/*metabolism
MH  - Serum Albumin/*analysis/chemistry/*metabolism
MH  - Spectrometry, Fluorescence
EDAT- 2007/09/05 09:00
MHDA- 2008/04/25 09:00
CRDT- 2007/09/05 09:00
PHST- 2007/09/05 09:00 [pubmed]
PHST- 2008/04/25 09:00 [medline]
PHST- 2007/09/05 09:00 [entrez]
AID - 10.1002/bio.1001 [doi]
PST - ppublish
SO  - Luminescence. 2007 Nov-Dec;22(6):559-66. doi: 10.1002/bio.1001.